User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/03 - Revision history

Mary Mendoza: /* Kinetics of AuNP (Citrate) */

Mary Mendoza — Tue, 11 Dec 2012 20:49:31 GMT

Kinetics of AuNP (Citrate)

←Older revision		Revision as of 20:49, 11 December 2012
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-	* Now that the optimal amount of HRP was found, the reaction of AAP and hydrogen peroxide with HRP was tested if this was affected by the addition of Au/Citrate. The first run is HRP alone with the reaction followed by the addition of Au/Citrate.	+
		+	* Now that the optimal amount of HRP was found, the reaction of AAP and hydrogen peroxide with HRP was tested if this was affected by the addition of Au/Citrate. The first run is HRP alone with the reaction followed by the addition of Au/Citrate as shown below.
		+	* Au/Citrate was made from 20 mL of 1 mM HAuCl<sub>4</sub> with 20 mL of 1% citrate. The Au/Citrate was added in multiple of 20. Each 20 μL is equivalent to 2%.

Mary Mendoza: /* Kinetics of AuNP (Citrate) */

Mary Mendoza — Tue, 11 Dec 2012 20:45:24 GMT

Kinetics of AuNP (Citrate)

←Older revision		Revision as of 20:45, 11 December 2012
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	[[Image:Aucit.png]]		[[Image:Aucit.png]]
		+
		+
		+
		+	* The amount and concentration of reactants are shown below.
		+
		+	{\| {{table}}
		+	\| align="center" style="background:#f0f0f0;"\|'''Trials'''
		+	\| align="center" style="background:#f0f0f0;"\|''''''
		+	\| align="center" style="background:#f0f0f0;"\|''''''
		+	\| align="center" style="background:#f0f0f0;"\|''''''
		+	\| align="center" style="background:#f0f0f0;"\|''''''
		+	\| align="center" style="background:#f0f0f0;"\|''''''
		+	\| align="center" style="background:#f0f0f0;"\|''''''
		+	\|-
		+	\| Cuvet\|\|hydrogen peroxide concentration (mM)\|\|Iodophenol in DMSO concentration (μM)\|\|AAP Concentration (μM)\|\|HRP concentration (μM)\|\|μL of gold np added\|\|μL of water added
		+	\|-
		+	\| 1( standard)\|\|1.7\|\|18\|\|156.25\|\|3.45\|\|0\|\|630
		+	\|-
		+	\| 1\|\|1.7\|\|18\|\|156.25\|\|3.45\|\|20\|\|610
		+	\|-
		+	\| 2\|\|1.7\|\|18\|\|156.25\|\|3.45\|\|40\|\|590
		+	\|-
		+	\| 3\|\|1.7\|\|18\|\|156.25\|\|3.45\|\|60\|\|570
		+	\|-
		+	\| 4\|\|1.7\|\|18\|\|156.25\|\|3.45\|\|80\|\|550
		+	\|-
		+	\| 5\|\|1.7\|\|18\|\|156.25\|\|3.45\|\|100\|\|530
		+	\|-
		+	\| Total volume added\|\|750 μL\|\|10 μL\|\|35 μL\|\|33 μL\|\|\|\|
		+	\|}
		+

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Mary Mendoza: /* Kinetics of AuNP (Citrate) */

Mary Mendoza — Tue, 11 Dec 2012 20:42:09 GMT

Kinetics of AuNP (Citrate)

←Older revision		Revision as of 20:42, 11 December 2012
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	==Kinetics of AuNP (Citrate)==		==Kinetics of AuNP (Citrate)==
	* As mentioned in the previous entry of [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02\|Oct. 2]], there was difficulty in finding the appropriate ratio between AAP and HRP.		* As mentioned in the previous entry of [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02\|Oct. 2]], there was difficulty in finding the appropriate ratio between AAP and HRP.
-	* ~~Trial~~ and error was done to find a linear trend for the absorbance of HRP. The calculated volume was 22 μL. The optimal volume for HRP was found at 33 μL. This can be seen on the graph below.	+	* Using the Kinetics program, trial and error was done to find a linear trend for the absorbance of HRP. The calculated volume was 22 μL. The optimal volume for HRP was found at 33 μL. This can be seen on the graph below.

	[[Image:HRP.png]]		[[Image:HRP.png]]


		+	* Now that the optimal amount of HRP was found, the reaction of AAP and hydrogen peroxide with HRP was tested if this was affected by the addition of Au/Citrate. The first run is HRP alone with the reaction followed by the addition of Au/Citrate.
		+
		+
		+	[[Image:Aucit.png]]

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Mary Mendoza: /* Kinetics of AuNP (Citrate) */

Mary Mendoza — Tue, 11 Dec 2012 20:38:44 GMT

Kinetics of AuNP (Citrate)

←Older revision		Revision as of 20:38, 11 December 2012
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	==Kinetics of AuNP (Citrate)==		==Kinetics of AuNP (Citrate)==
	* As mentioned in the previous entry of [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02\|Oct. 2]], there was difficulty in finding the appropriate ratio between AAP and HRP.		* As mentioned in the previous entry of [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02\|Oct. 2]], there was difficulty in finding the appropriate ratio between AAP and HRP.
-	* ~~As suggested by Dr. Miller,~~ the ~~group addressed the issue by observing the problem in terms of molarity instead~~ of volume.	+	* Trial and error was done to find a linear trend for the absorbance of HRP. The calculated volume was 22 μL. The optimal volume for HRP was found at 33 μL. This can be seen on the graph below.
-	* By comparing the ~~molarity of the reactants,~~	+
		+	[[Image:HRP.png]]

-	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:41, 7 October 2012 (EDT)''':what did you do? concnetrations? runs? then worry about adding the analysis.

Mary Mendoza: /* Kinetics of AuNP (Citrate) */

Mary Mendoza — Tue, 09 Oct 2012 02:26:36 GMT

Kinetics of AuNP (Citrate)

←Older revision		Revision as of 02:26, 9 October 2012
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	* As mentioned in the previous entry of [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02\|Oct. 2]], there was difficulty in finding the appropriate ratio between AAP and HRP.		* As mentioned in the previous entry of [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02\|Oct. 2]], there was difficulty in finding the appropriate ratio between AAP and HRP.
	* As suggested by Dr. Miller, the group addressed the issue by observing the problem in terms of molarity instead of volume.		* As suggested by Dr. Miller, the group addressed the issue by observing the problem in terms of molarity instead of volume.
-	* By comparing the molarity of the reactants,	+	* By comparing the molarity of the reactants,

	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:41, 7 October 2012 (EDT)''':what did you do? concnetrations? runs? then worry about adding the analysis.		*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:41, 7 October 2012 (EDT)''':what did you do? concnetrations? runs? then worry about adding the analysis.

Mary Mendoza: /* Kinetics of AuNP (Citrate) */

Mary Mendoza — Tue, 09 Oct 2012 00:43:30 GMT

Kinetics of AuNP (Citrate)

←Older revision		Revision as of 00:43, 9 October 2012
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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==Kinetics of AuNP (Citrate)==		==Kinetics of AuNP (Citrate)==
-	* As mentioned in the previous entry of [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02\|Oct. 2]], there was difficulty in	+	* As mentioned in the previous entry of [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02\|Oct. 2]], there was difficulty in finding the appropriate ratio between AAP and HRP.
		+	* As suggested by Dr. Miller, the group addressed the issue by observing the problem in terms of molarity instead of volume.
		+	* By comparing the molarity of the reactants,

	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:41, 7 October 2012 (EDT)''':what did you do? concnetrations? runs? then worry about adding the analysis.		*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:41, 7 October 2012 (EDT)''':what did you do? concnetrations? runs? then worry about adding the analysis.

Mary Mendoza: /* Kinetics of AuNP (Citrate) */

Mary Mendoza — Tue, 09 Oct 2012 00:02:39 GMT

Kinetics of AuNP (Citrate)

←Older revision		Revision as of 00:02, 9 October 2012
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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==Kinetics of AuNP (Citrate)==		==Kinetics of AuNP (Citrate)==
-	* As mentioned in the previous entry [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02\|Oct. 2]]	+	* As mentioned in the previous entry of [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02\|Oct. 2]], there was difficulty in

	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:41, 7 October 2012 (EDT)''':what did you do? concnetrations? runs? then worry about adding the analysis.		*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:41, 7 October 2012 (EDT)''':what did you do? concnetrations? runs? then worry about adding the analysis.

Mary Mendoza: /* Kinetics of AuNP (Citrate) */

Mary Mendoza — Tue, 09 Oct 2012 00:01:20 GMT

Kinetics of AuNP (Citrate)

←Older revision		Revision as of 00:01, 9 October 2012
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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==Kinetics of AuNP (Citrate)==		==Kinetics of AuNP (Citrate)==
-	* As mentioned in the previous entry [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02 Oct. 2]]	+	* As mentioned in the previous entry [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02\|Oct. 2]]

	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:41, 7 October 2012 (EDT)''':what did you do? concnetrations? runs? then worry about adding the analysis.		*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:41, 7 October 2012 (EDT)''':what did you do? concnetrations? runs? then worry about adding the analysis.

Mary Mendoza: /* Kinetics of AuNP (Citrate) */

Mary Mendoza — Tue, 09 Oct 2012 00:01:03 GMT

Kinetics of AuNP (Citrate)

←Older revision		Revision as of 00:01, 9 October 2012
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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==Kinetics of AuNP (Citrate)==		==Kinetics of AuNP (Citrate)==
-	* ~~After evaluating~~	+	* As mentioned in the previous entry [[User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02 Oct. 2]]

	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:41, 7 October 2012 (EDT)''':what did you do? concnetrations? runs? then worry about adding the analysis.		*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:41, 7 October 2012 (EDT)''':what did you do? concnetrations? runs? then worry about adding the analysis.

Abigail E. Miller: /* Kinetics of AuNP (Citrate) */

Abigail E. Miller — Sun, 07 Oct 2012 14:41:29 GMT

Kinetics of AuNP (Citrate)

←Older revision		Revision as of 14:41, 7 October 2012
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	==Kinetics of AuNP (Citrate)==		==Kinetics of AuNP (Citrate)==
	* After evaluating		* After evaluating
		+
		+	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:41, 7 October 2012 (EDT)''':what did you do? concnetrations? runs? then worry about adding the analysis.