User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Mary Mendoza (talk | contribs) |
Mary Mendoza (talk | contribs) |
||
Line 55: | Line 55: | ||
==HRP Assay Figures== | ==HRP Assay Figures== | ||
* Charts embedded below | * Charts embedded below represent the HRP Assay runs that have varying concentrations of HRP and AAP. The variations in the physical condition of the surrounding environment were done to investigate the appropriate ratio between HRP and AAP. | ||
[[Image:HRP10022012.png]] | [[Image:HRP10022012.png]] | ||
* Runs 1, 3, and 4 reflect absorbance trends where the reaction had a low concentration of HRP. | * Runs 1, 3, and 4 reflect absorbance trends where the reaction had a low concentration of HRP. | ||
* Run 2 shows the reaction where there was an error in calculating the proper volume for the HRP and AAP ratio. The group added 50 μL of HRP against a very low concentration of AAP. | * Run 2 shows the reaction where there was an error in calculating the proper volume for the HRP and AAP ratio. The group added 50 μL of HRP against a very low concentration of AAP. | ||
* Run 5 is the replicated absorbance from MDK using the | * Run 5 is the replicated absorbance from MDK using the standard condition where the volume of AAP is 700 μL and HRP is 50 μL. | ||
[[Image:DviewHRP10022012.png]] | [[Image:DviewHRP10022012.png]] | ||
Revision as of 17:09, 8 October 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||
Preparation of the Reactants of HRP Oxidase Assay
0.020 mol of Na2HPO4 × [math]\displaystyle{ \frac{268.07g}{1mol} }[/math] of Na2HPO4 = 53.61 g of Na2HPO4
HRP Assay Figures
|