User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02: Difference between revisions
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==Preparation of the Reactants of HRP Oxidase Assay== | ==Preparation of the Reactants of HRP Oxidase Assay== | ||
* The reactants listed in [http://openwetware.org/wiki/Image:3760-Protocol_HRP_AbsorbanceAssay1.pdf HRP Oxidase Assay] | * The reactants listed in [http://openwetware.org/wiki/Image:3760-Protocol_HRP_AbsorbanceAssay1.pdf HRP Oxidase Assay] were weighed out. | ||
* The phosphate buffer was made with white granules of sodium phosphate dibasic (FW 268.07, heptahydrate) instead of potassium phosphate. The substitution is not detrimental to the procedure since the ion of interest is the phosphate group. | |||
* Calculations are listed below: | |||
0.020 mol of Na<sub>2</sub>HPO<sub>4</sub> <math>\frac{268.07g}{1mol}</math> | |||
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Revision as of 18:56, 6 October 2012
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Preparation of the Reactants of HRP Oxidase Assay
0.020 mol of Na2HPO4 [math]\displaystyle{ \frac{268.07g}{1mol} }[/math] | |
