User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02: Difference between revisions
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* Placed the settings of the spectrophotometer on kinetics, at 25°C, 510 nm from the methods function. | * Placed the settings of the spectrophotometer on kinetics, at 25°C, 510 nm from the methods function. | ||
* In reference to the document of [http://openwetware.org/wiki/Image:HRP_Assays.docx Novy, Patel, and Wang], the group has chosen to prepare the sample solution with the most linearity. The sample solution has the following concentration of reactants. | * In reference to the document of [http://openwetware.org/wiki/Image:HRP_Assays.docx Novy, Patel, and Wang (MDK)], the group has chosen to prepare the sample solution with the most linearity. The sample solution has the following concentration of reactants listed in the table below. | ||
* There was difficulty of replicating the sample concentration from MDK. A run using a volume of 35 μL of AAP and 2.5 μL of HRP had a flat signal indicating the possibilities of having no or slow reaction occurring. | |||
{| {{table}} | {| {{table}} |
Revision as of 21:53, 7 October 2012
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Preparation of the Reactants of HRP Oxidase Assay
0.020 mol of Na2HPO4 × [math]\displaystyle{ \frac{268.07g}{1mol} }[/math] of Na2HPO4 = 53.61 g of Na2HPO4
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