User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02: Difference between revisions
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* Transferred the reactants to a plastic disposable sample cell using a micropipet. NOTE: The HRP was added last when the sample was already inside the chamber. The addition of HRP initiates the reaction. | * Transferred the reactants to a plastic disposable sample cell using a micropipet. NOTE: The HRP was added last when the sample was already inside the chamber. The addition of HRP initiates the reaction. | ||
* The solution sample appeared as clear and colorless liquid solution before the reaction. At the end of the reaction, the sample was ruby-red at the top of the sample cell and clear and colorless at the bottom. There is a defined separation between the two color phases. | |||
* There was difficulty of replicating the sample concentration from MDK. A run using a volume of 35 μL of AAP and 2.5 μL of HRP had a flat signal indicating the possibilities of having no or slow reaction occurring. Increasing the concentration of HRP made the reaction occur at an instant. | * There was difficulty of replicating the sample concentration from MDK. A run using a volume of 35 μL of AAP and 2.5 μL of HRP had a flat signal indicating the possibilities of having no or slow reaction occurring. Increasing the concentration of HRP made the reaction occur at an instant. | ||
* For the following day, the agenda would be to ascertain the appropriate volume of AAP while keeping the same concentration. | |||
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Revision as of 22:31, 7 October 2012
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Preparation of the Reactants of HRP Oxidase Assay
0.020 mol of Na2HPO4 × [math]\displaystyle{ \frac{268.07g}{1mol} }[/math] of Na2HPO4 = 53.61 g of Na2HPO4
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