User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02

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(→Preparation of the Reactants of HRP Oxidase Assay)
(→Preparation of the Reactants of HRP Oxidase Assay)
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* The required weight for 4-iodophenol is 0.810 g to make 0.0025 M. The actual weighed amount was 0.8109 g in 700 μM
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* The required weight for 4-iodophenol is 0.810 g to make 0.0025 M. The actual weighed amount was 0.8109 g. This amount is dissolved 700 μM dimethyl sulfoxide (DMSO, polar aprotic solvent) due to its immiscibility in water.
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* The
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* Weighed out 1 mg of the brown granu

Revision as of 23:22, 7 October 2012

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Preparation of the Reactants of HRP Oxidase Assay

  • The reactants listed in HRP Oxidase Assay were weighed out.
  • The phosphate buffer was made with white granules of sodium phosphate dibasic (FW 268.07, heptahydrate) instead of potassium phosphate. The substitution is not detrimental to the procedure since the ion of interest is the phosphate group. The total volume chosen for the buffer is 60 mL.
  • Calculation for obtaining Na2HPO4:

0.020 mol of Na2HPO4 × \frac{268.07g}{1mol} of Na2HPO4 = 53.61 g of Na2HPO4


x60 mL = \frac{.060 L * 53. 61 g}{1L} = 3.22 g of Na2HPO4


  • The required weight for 4-iodophenol is 0.810 g to make 0.0025 M. The actual weighed amount was 0.8109 g. This amount is dissolved 700 μM dimethyl sulfoxide (DMSO, polar aprotic solvent) due to its immiscibility in water.
  • Weighed out 1 mg of the brown granu


  • Abigail E. Miller 10:40, 7 October 2012 (EDT):you are missing a bunch or reactants and your data from this date. show what happened and what problem you had to assess for the next day.



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