# User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02

(Difference between revisions)
 Revision as of 18:36, 7 October 2012 (view source) (→Preparation of the Reactants of HRP Oxidase Assay)← Previous diff Revision as of 18:49, 7 October 2012 (view source) (→Preparation of the Reactants of HRP Oxidase Assay)Next diff → Line 17: Line 17: - * The required weight for 4-aminoantipyrine is 0.810 g. The actual weighed amount was 0.8109 g of the yellow granular solid. + * The required weight for 4-aminoantipyrine is 0.810 g. The actual weighed amount was 0.8109 g of the yellow granular solid. This is immiscible in water + - *

## Revision as of 18:49, 7 October 2012

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## Preparation of the Reactants of HRP Oxidase Assay

• The reactants listed in HRP Oxidase Assay were weighed out.
• The phosphate buffer was made with white granules of sodium phosphate dibasic (FW 268.07, heptahydrate) instead of potassium phosphate. The substitution is not detrimental to the procedure since the ion of interest is the phosphate group. The total volume chosen for the buffer is 60 mL.
• Calculation for obtaining Na2HPO4:

0.020 mol of Na2HPO4 × $\frac{268.07g}{1mol}$ of Na2HPO4 = 53.61 g of Na2HPO4

x60 mL = $\frac{.060 L * 53. 61 g}{1L}$ = 3.22 g of Na2HPO4

• The required weight for 4-aminoantipyrine is 0.810 g. The actual weighed amount was 0.8109 g of the yellow granular solid. This is immiscible in water

• Abigail E. Miller 10:40, 7 October 2012 (EDT):you are missing a bunch or reactants and your data from this date. show what happened and what problem you had to assess for the next day.