User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/10/02

(Difference between revisions)

Revision as of 22:37, 6 October 2012

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The reactants listed in HRP Oxidase Assay were weighed out.
The phosphate buffer was made with white granules of sodium phosphate dibasic (FW 268.07, heptahydrate) instead of potassium phosphate. The substitution is not detrimental to the procedure since the ion of interest is the phosphate group. The total volume chosen for the buffer is 60 mL.
Calculation for obtaining Na₂HPO₄

0.020 mol of Na₂HPO₄ × $\frac{268.07g}{1mol}$ of Na₂HPO₄ = 53.61 g of Na₂HPO₄

x_{60 mL} = $\frac{.060 L * 53. 61 g}{1L}$ = 3.22 g of Na₂HPO₄

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 * The reactants listed in [http://openwetware.org/wiki/Image:3760-Protocol_HRP_AbsorbanceAssay1.pdf HRP Oxidase Assay] were weighed out.
 * The phosphate buffer was made with white granules of sodium phosphate dibasic (FW 268.07, heptahydrate) instead of potassium phosphate. The substitution is not detrimental to the procedure since the ion of interest is the phosphate group. The total volume chosen for the buffer is 60 mL.
-* Calculations are listed below:
+* Calculation for obtaining Na<sub>2</sub>HPO<sub>4</sub>
 .020 mol of Na<sub>2</sub>HPO<sub>4</sub> × <math>\frac{268.07g}{1mol}</math> of Na<sub>2</sub>HPO<sub>4</sub> = 53.61 g of Na<sub>2</sub>HPO<sub>4</sub>
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 x<sub>60 mL</sub> = <math>\frac{.060 L * 53. 61 g}{1L}</math> = 3.22 g of Na<sub>2</sub>HPO<sub>4</sub>
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