User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/26: Difference between revisions
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* From the readings, the detection indicates that the absorbance is at 280; indicative of the presence of Histidine. However, the absorbance at 280 is not exclusive to Histidine alone but also corresponds to Tryptophan, Tyrosine, and Phenylalanine. | * From the readings, the detection indicates that the absorbance is at 280; indicative of the presence of Histidine. However, the absorbance at 280 is not exclusive to Histidine alone but also corresponds to Tryptophan, Tyrosine, and Phenylalanine. | ||
* The conductivity reading is sensitive to the amount of salt in the buffer. | * The conductivity reading is sensitive to the amount of salt in the buffer. | ||
* On the dialog box, pump > gradient > B > 100%. | * On the dialog box, pump > gradient > B > 100%. | ||
* Once the peak levels back to baseline, pump > gradient > B > 0%. This washes the system with A. | |||
* At Flowpath > injection valve > position > inject. Switch to position 1. This bypasses the | |||
Revision as of 04:13, 5 October 2012
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Purification of Proteins
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