User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/25

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Lysis of transformed E. coli

  • To retrieve the adenosine deaminase (ADA) from E.coli, the cell must be lysed. By lysing the cell, the cytoplasm will no longer isolate the macromolecules, such as the ADA for this instance, from the extracellular environment.
  • There are different methods by which the cell can be lysed. These methods include heat shock, blender polytron, homogenization etc. For this procedure, sonication will be used to lyse the cell.
  • Cell lysis is feasible through sonication by exposing the cells to high frequency sound waves. These sound waves shear the membrane of the cells.



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