User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/25 - Revision history

Mary Mendoza: /* Filtration of the Supernatant */

2012-12-07T13:39:16Z

Filtration of the Supernatant

←Older revision		Revision as of 13:39, 7 December 2012
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	* The current pellet was left untouched in the centrifuge tubes. This are discarded material that contains disrupted cell organelles and other cellular material.		* The current pellet was left untouched in the centrifuge tubes. This are discarded material that contains disrupted cell organelles and other cellular material.

-
-	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:36, 7 October 2012 (EDT)''':very nice, please credit any images you use by citing and link back to any images you borrow. If you created it yourself, please cite that.

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Mary Mendoza: /* Filtration of Buffers */

2012-12-07T13:38:41Z

Filtration of Buffers

←Older revision		Revision as of 13:38, 7 December 2012
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	==Filtration of Buffers==		==Filtration of Buffers==
-	[[Image:Bufpix.jpg\|thumb\|right\|Assembly of Membrane Filtration]]	+	[[Image:Bufpix.jpg\|thumb\|right\|Assembly of Membrane Filtration Image Source: http://academic.pgcc.edu/~kroberts/Lecture/Chapter%206/counting.html]]
	* The assembly shown in the picture on the right was followed with an additional clip that would hold the filter tightly to the flask. The sidearm of the 1000 mL flask was connected to a Welch vacuum pump.		* The assembly shown in the picture on the right was followed with an additional clip that would hold the filter tightly to the flask. The sidearm of the 1000 mL flask was connected to a Welch vacuum pump.
	* The membrane filter had a diameter of 47mm with a 450 nm pore diameter.		* The membrane filter had a diameter of 47mm with a 450 nm pore diameter.

Abigail E. Miller at 14:36, 7 October 2012

2012-10-07T14:36:25Z

←Older revision		Revision as of 14:36, 7 October 2012
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-		+	*'''[[User:Abigail E. Miller\|Abigail E. Miller]] 10:36, 7 October 2012 (EDT)''':very nice, please credit any images you use by citing and link back to any images you borrow. If you created it yourself, please cite that.

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Mary Mendoza: /* Filtration of Buffers */

2012-10-05T09:42:51Z

Filtration of Buffers

←Older revision		Revision as of 09:42, 5 October 2012
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	* The collected filtrate of the binding buffer was poured into a sterilized glass bottle. This was followed by the pouring of the elution buffer into the filtering system. The filtrate for the elution buffer was also poured into a sterilized glass bottle.		* The collected filtrate of the binding buffer was poured into a sterilized glass bottle. This was followed by the pouring of the elution buffer into the filtering system. The filtrate for the elution buffer was also poured into a sterilized glass bottle.
	* The bottles were capped with aluminum foil tops and refrigerated. The buffers were clear, colorless liquid solution before and after filtration.		* The bottles were capped with aluminum foil tops and refrigerated. The buffers were clear, colorless liquid solution before and after filtration.
		+	* The filtrates were poured into their respective falcon tubes and refrigerated.

	==Filtration of the Supernatant==		==Filtration of the Supernatant==

Mary Mendoza: /* Filtration of the Supernatant */

2012-10-05T09:40:15Z

Filtration of the Supernatant

←Older revision		Revision as of 09:40, 5 October 2012
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	==Filtration of the Supernatant==		==Filtration of the Supernatant==
		+	* The centrifuge tubes were taken out from the chamber. The supernatant of the tubes were poured into the filtering system. Sample 1 remained isolated from sample 2 which contained pellets 2, 3, and 4 from last week.
		+	* The current pellet was left untouched in the centrifuge tubes. This are discarded material that contains disrupted cell organelles and other cellular material.
		+

Mary Mendoza: /* Filtration of Buffers */

2012-10-05T09:35:13Z

Filtration of Buffers

←Older revision		Revision as of 09:35, 5 October 2012
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	* The first pour was done with the binding buffer. This was done to ensure that the buffer with the lower imidazole content (binding) would not be contaminated with the buffer that had the higher imidazole content (elution).		* The first pour was done with the binding buffer. This was done to ensure that the buffer with the lower imidazole content (binding) would not be contaminated with the buffer that had the higher imidazole content (elution).
	* The collected filtrate of the binding buffer was poured into a sterilized glass bottle. This was followed by the pouring of the elution buffer into the filtering system. The filtrate for the elution buffer was also poured into a sterilized glass bottle.		* The collected filtrate of the binding buffer was poured into a sterilized glass bottle. This was followed by the pouring of the elution buffer into the filtering system. The filtrate for the elution buffer was also poured into a sterilized glass bottle.
-	* The bottles were capped with aluminum foil tops and refrigerated.	+	* The bottles were capped with aluminum foil tops and refrigerated. The buffers were clear, colorless liquid solution before and after filtration.

	==Filtration of the Supernatant==		==Filtration of the Supernatant==

Mary Mendoza: /* Filtration of Buffers */

2012-10-05T09:34:05Z

Filtration of Buffers

←Older revision		Revision as of 09:34, 5 October 2012
Line 26:		Line 26:
	* The collected filtrate of the binding buffer was poured into a sterilized glass bottle. This was followed by the pouring of the elution buffer into the filtering system. The filtrate for the elution buffer was also poured into a sterilized glass bottle.		* The collected filtrate of the binding buffer was poured into a sterilized glass bottle. This was followed by the pouring of the elution buffer into the filtering system. The filtrate for the elution buffer was also poured into a sterilized glass bottle.
	* The bottles were capped with aluminum foil tops and refrigerated.		* The bottles were capped with aluminum foil tops and refrigerated.
		+
		+	==Filtration of the Supernatant==
		+
		+

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Mary Mendoza: /* Filtration of Buffers */

2012-10-05T09:29:32Z

Filtration of Buffers

←Older revision		Revision as of 09:29, 5 October 2012
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	* The pump was turned on; the knob was unscrewed. The setting was set to 60 cmHg vacuum. This setting was chosen at random.		* The pump was turned on; the knob was unscrewed. The setting was set to 60 cmHg vacuum. This setting was chosen at random.
	* The first pour was done with the binding buffer. This was done to ensure that the buffer with the lower imidazole content (binding) would not be contaminated with the buffer that had the higher imidazole content (elution).		* The first pour was done with the binding buffer. This was done to ensure that the buffer with the lower imidazole content (binding) would not be contaminated with the buffer that had the higher imidazole content (elution).
-	* The collected filtrate of the binding buffer was poured into a sterilized glass bottle. This was followed by the pouring of the elution buffer into the filtering system.	+	* The collected filtrate of the binding buffer was poured into a sterilized glass bottle. This was followed by the pouring of the elution buffer into the filtering system. The filtrate for the elution buffer was also poured into a sterilized glass bottle.
		+	* The bottles were capped with aluminum foil tops and refrigerated.

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Mary Mendoza: /* Filtration of Buffers */

2012-10-05T09:27:59Z

Filtration of Buffers

←Older revision		Revision as of 09:27, 5 October 2012
Line 23:		Line 23:
	* The membrane filter had a diameter of 47mm with a 450 nm pore diameter.		* The membrane filter had a diameter of 47mm with a 450 nm pore diameter.
	* The pump was turned on; the knob was unscrewed. The setting was set to 60 cmHg vacuum. This setting was chosen at random.		* The pump was turned on; the knob was unscrewed. The setting was set to 60 cmHg vacuum. This setting was chosen at random.
-	* The first pour was done with the binding buffer. This was done to ensure that the buffer with the lower imidazole content (binding) would not be contaminated with the buffer that had the higher imidazole content (elution).	+	* The first pour was done with the binding buffer. This was done to ensure that the buffer with the lower imidazole content (binding) would not be contaminated with the buffer that had the higher imidazole content (elution).
-		+	* The collected filtrate of the binding buffer was poured into a sterilized glass bottle. This was followed by the pouring of the elution buffer into the filtering system.

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Mary Mendoza: /* Filtration of Buffers */

2012-10-05T09:23:51Z

Filtration of Buffers

←Older revision		Revision as of 09:23, 5 October 2012
Line 23:		Line 23:
	* The membrane filter had a diameter of 47mm with a 450 nm pore diameter.		* The membrane filter had a diameter of 47mm with a 450 nm pore diameter.
	* The pump was turned on; the knob was unscrewed. The setting was set to 60 cmHg vacuum. This setting was chosen at random.		* The pump was turned on; the knob was unscrewed. The setting was set to 60 cmHg vacuum. This setting was chosen at random.
		+	* The first pour was done with the binding buffer. This was done to ensure that the buffer with the lower imidazole content (binding) would not be contaminated with the buffer that had the higher imidazole content (elution).