User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/25: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Mary Mendoza (talk | contribs) |
Mary Mendoza (talk | contribs) |
||
Line 24: | Line 24: | ||
* The pump was turned on; the knob was unscrewed. The setting was set to 60 cmHg vacuum. This setting was chosen at random. | * The pump was turned on; the knob was unscrewed. The setting was set to 60 cmHg vacuum. This setting was chosen at random. | ||
* The first pour was done with the binding buffer. This was done to ensure that the buffer with the lower imidazole content (binding) would not be contaminated with the buffer that had the higher imidazole content (elution). | * The first pour was done with the binding buffer. This was done to ensure that the buffer with the lower imidazole content (binding) would not be contaminated with the buffer that had the higher imidazole content (elution). | ||
* The collected filtrate of the binding buffer was poured into a sterilized glass bottle. This was followed by the pouring of the elution buffer into the filtering system. | * The collected filtrate of the binding buffer was poured into a sterilized glass bottle. This was followed by the pouring of the elution buffer into the filtering system. The filtrate for the elution buffer was also poured into a sterilized glass bottle. | ||
* The bottles were capped with aluminum foil tops and refrigerated. | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 02:29, 5 October 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Lysis of transformed E. coli
Filtration of Buffers
|