User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/25: Difference between revisions
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* Sample 1 was uncapped and inserted through the nozzle of the sonicator for 30 sec. followed by a 30 sec. soak in an ice bath. The nozzle is wiped off with kim wipes. Cooling the sample in an ice bath is necessary to prevent the denaturing of proteins. Denaturation can occur due to the heat generated by the high frequency sound waves of the sonicator. | * Sample 1 was uncapped and inserted through the nozzle of the sonicator for 30 sec. followed by a 30 sec. soak in an ice bath. The nozzle is wiped off with kim wipes. Cooling the sample in an ice bath is necessary to prevent the denaturing of proteins. Denaturation can occur due to the heat generated by the high frequency sound waves of the sonicator. | ||
* While sample 1 is in the ice bath, sample 2 was inserted through the nozzle. These steps were repeated two more times with a total repetition of 3 for each sample. | * While sample 1 is in the ice bath, sample 2 was inserted through the nozzle. These steps were repeated two more times with a total repetition of 3 for each sample. | ||
* Upon finishing sonication of cells, the samples were transferred to centrifuge tubes. The rotor chosen for the tubes is the make of Fiberlite (Piramon Technologies Inc.) rotor F21-8x50y max. 21,000 rpm. | |||
* The centrifuge settings were fixed on 18,000 rpm at 4°C for 2 h. | |||
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Revision as of 15:21, 25 September 2012
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Lysis of transformed E. coli
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