User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I/2012/09/25: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Filtration of Buffers== | ==Filtration of Buffers== | ||
[[Image:Bufpix.jpg|thumb|right|Assembly of Membrane Filtration]] | [[Image:Bufpix.jpg|thumb|right|Assembly of Membrane Filtration Image Source: http://academic.pgcc.edu/~kroberts/Lecture/Chapter%206/counting.html]] | ||
* The assembly shown in the picture on the right was followed with an additional clip that would hold the filter tightly to the flask. The sidearm of the 1000 mL flask was connected to a Welch vacuum pump. | * The assembly shown in the picture on the right was followed with an additional clip that would hold the filter tightly to the flask. The sidearm of the 1000 mL flask was connected to a Welch vacuum pump. | ||
* The membrane filter had a diameter of 47mm with a 450 nm pore diameter. | * The membrane filter had a diameter of 47mm with a 450 nm pore diameter. | ||
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* The collected filtrate of the binding buffer was poured into a sterilized glass bottle. This was followed by the pouring of the elution buffer into the filtering system. The filtrate for the elution buffer was also poured into a sterilized glass bottle. | * The collected filtrate of the binding buffer was poured into a sterilized glass bottle. This was followed by the pouring of the elution buffer into the filtering system. The filtrate for the elution buffer was also poured into a sterilized glass bottle. | ||
* The bottles were capped with aluminum foil tops and refrigerated. The buffers were clear, colorless liquid solution before and after filtration. | * The bottles were capped with aluminum foil tops and refrigerated. The buffers were clear, colorless liquid solution before and after filtration. | ||
* The filtrates were poured into their respective falcon tubes and refrigerated. | |||
==Filtration of the Supernatant== | ==Filtration of the Supernatant== | ||
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* The current pellet was left untouched in the centrifuge tubes. This are discarded material that contains disrupted cell organelles and other cellular material. | * The current pellet was left untouched in the centrifuge tubes. This are discarded material that contains disrupted cell organelles and other cellular material. | ||
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Latest revision as of 22:04, 26 September 2017
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Lysis of transformed E. coli
Filtration of Buffers
Filtration of the Supernatant
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