User:Mary Mendoza/Notebook/CHEM 571 Experimental Biological Chemistry I - Revision history

Mary Mendoza: /* Abstract */

Mary Mendoza — Tue, 11 Dec 2012 21:21:49 GMT

Abstract

←Older revision		Revision as of 21:21, 11 December 2012
Line 20:		Line 20:
	* The reaction of Aminoantipyrine and hydrogen peroxide with HRP was studied before moving on to chemiluminescence.		* The reaction of Aminoantipyrine and hydrogen peroxide with HRP was studied before moving on to chemiluminescence.
	* For the chemiluminescence reaction, aminoantipyrine was replaced with aminophthalhydrazide (luminol). The replacement produces the fluorescent aminophthalate.		* For the chemiluminescence reaction, aminoantipyrine was replaced with aminophthalhydrazide (luminol). The replacement produces the fluorescent aminophthalate.
		+	* At the end of the semester, experiments were conducted on the synthesis of Au/lysozyme and Au/ADA nanoparticles.

	==Notes==		==Notes==

Mary Mendoza: /* Abstract */

Mary Mendoza — Tue, 11 Dec 2012 21:20:41 GMT

Abstract

←Older revision		Revision as of 21:20, 11 December 2012
Line 19:		Line 19:
	* Once ionic strength was accomplished, the experiment shifted through ADA expression from transformed ''E.coli''. The collected proteins were purified by Fast Protein Liquid Chromatography and stored.		* Once ionic strength was accomplished, the experiment shifted through ADA expression from transformed ''E.coli''. The collected proteins were purified by Fast Protein Liquid Chromatography and stored.
	* The reaction of Aminoantipyrine and hydrogen peroxide with HRP was studied before moving on to chemiluminescence.		* The reaction of Aminoantipyrine and hydrogen peroxide with HRP was studied before moving on to chemiluminescence.
		+	* For the chemiluminescence reaction, aminoantipyrine was replaced with aminophthalhydrazide (luminol). The replacement produces the fluorescent aminophthalate.

	==Notes==		==Notes==

Mary Mendoza: /* Abstract */

Mary Mendoza — Tue, 11 Dec 2012 21:19:12 GMT

Abstract

←Older revision		Revision as of 21:19, 11 December 2012
Line 18:		Line 18:
	* AuNP were studied in various ways. After the synthesis of Au/BSA, addition of various pH buffers and concentration of tris(hydroxymethyl)aminomethane were conducted. Data was collected and evaluated through UV-visible scans.		* AuNP were studied in various ways. After the synthesis of Au/BSA, addition of various pH buffers and concentration of tris(hydroxymethyl)aminomethane were conducted. Data was collected and evaluated through UV-visible scans.
	* Once ionic strength was accomplished, the experiment shifted through ADA expression from transformed ''E.coli''. The collected proteins were purified by Fast Protein Liquid Chromatography and stored.		* Once ionic strength was accomplished, the experiment shifted through ADA expression from transformed ''E.coli''. The collected proteins were purified by Fast Protein Liquid Chromatography and stored.
		+	* The reaction of Aminoantipyrine and hydrogen peroxide with HRP was studied before moving on to chemiluminescence.

	==Notes==		==Notes==

Mary Mendoza: /* Abstract */

Mary Mendoza — Tue, 11 Dec 2012 21:15:52 GMT

Abstract

←Older revision		Revision as of 21:15, 11 December 2012
Line 15:		Line 15:
	==Abstract==		==Abstract==
	* In reference to the investigation of [http://pubs.acs.org/doi/abs/10.1021/jp110296y Bakshi, et al], bovine serum albumin (BSA) was found to form nanoparticles with gold (Au). BSA acts as a reducing agent by converting Au (III) into Au (0). With the application of heat, the BSA unfolds and its amino acid, cysteine, reacts with the hydrogen bonds of chloroauric acid, HAuCl<sub>4</sub>. This happens to induce the reduction of Au.		* In reference to the investigation of [http://pubs.acs.org/doi/abs/10.1021/jp110296y Bakshi, et al], bovine serum albumin (BSA) was found to form nanoparticles with gold (Au). BSA acts as a reducing agent by converting Au (III) into Au (0). With the application of heat, the BSA unfolds and its amino acid, cysteine, reacts with the hydrogen bonds of chloroauric acid, HAuCl<sub>4</sub>. This happens to induce the reduction of Au.
		+
		+	* AuNP were studied in various ways. After the synthesis of Au/BSA, addition of various pH buffers and concentration of tris(hydroxymethyl)aminomethane were conducted. Data was collected and evaluated through UV-visible scans.
		+	* Once ionic strength was accomplished, the experiment shifted through ADA expression from transformed ''E.coli''. The collected proteins were purified by Fast Protein Liquid Chromatography and stored.

	==Notes==		==Notes==

Mary Mendoza: /* Abstract */

Mary Mendoza — Mon, 10 Dec 2012 03:16:48 GMT

Abstract

←Older revision		Revision as of 03:16, 10 December 2012
Line 15:		Line 15:
	==Abstract==		==Abstract==
	* In reference to the investigation of [http://pubs.acs.org/doi/abs/10.1021/jp110296y Bakshi, et al], bovine serum albumin (BSA) was found to form nanoparticles with gold (Au). BSA acts as a reducing agent by converting Au (III) into Au (0). With the application of heat, the BSA unfolds and its amino acid, cysteine, reacts with the hydrogen bonds of chloroauric acid, HAuCl<sub>4</sub>. This happens to induce the reduction of Au.		* In reference to the investigation of [http://pubs.acs.org/doi/abs/10.1021/jp110296y Bakshi, et al], bovine serum albumin (BSA) was found to form nanoparticles with gold (Au). BSA acts as a reducing agent by converting Au (III) into Au (0). With the application of heat, the BSA unfolds and its amino acid, cysteine, reacts with the hydrogen bonds of chloroauric acid, HAuCl<sub>4</sub>. This happens to induce the reduction of Au.
-
-	* The agenda of this laboratory is to manipulate the two main factors, temperature and unfolding of BSA, (independent variables) that govern the formation of gold nanoparticles, AuNPs.
-
-	* The first 3 weeks of the experiment, from August 29 to September 12, concerns the experimentation on pH and ionic strength by which temperature of the Au/BSA solution and concentration of chloroauric acid is tested against tris buffer at different pH levels, in particular pH 8 and 10.

	==Notes==		==Notes==

Mary Mendoza: /* Notes */

Mary Mendoza — Tue, 09 Oct 2012 20:33:06 GMT

Notes

←Older revision		Revision as of 20:33, 9 October 2012
Line 23:		Line 23:
	* For each entry, headings are written in consecutive order of execution.		* For each entry, headings are written in consecutive order of execution.
	* Observations, significance of particular steps, and discussions are indicated within the procedure.		* Observations, significance of particular steps, and discussions are indicated within the procedure.
		+	* Water used for the entirety of the requirements in the experiment was de-ionized and distilled water. Also, the water is run through a current to ensure that there is no organic matter present.

Mary Mendoza: /* Notes */

Mary Mendoza — Fri, 05 Oct 2012 11:13:56 GMT

Notes

←Older revision		Revision as of 11:13, 5 October 2012
Line 22:		Line 22:
	==Notes==		==Notes==
	* For each entry, headings are written in consecutive order of execution.		* For each entry, headings are written in consecutive order of execution.
-	* Observations, significance of particular steps, and discussions are indicated in the procedure.	+	* Observations, significance of particular steps, and discussions are indicated within the procedure.

Mary Mendoza: /* Notes */

Mary Mendoza — Thu, 04 Oct 2012 22:25:12 GMT

Notes

←Older revision		Revision as of 22:25, 4 October 2012
Line 22:		Line 22:
	==Notes==		==Notes==
	* For each entry, headings are written in consecutive order of execution.		* For each entry, headings are written in consecutive order of execution.
-		+	* Observations, significance of particular steps, and discussions are indicated in the procedure.

Mary Mendoza: /* Notes */

Mary Mendoza — Thu, 04 Oct 2012 22:22:41 GMT

Notes

←Older revision		Revision as of 22:22, 4 October 2012
Line 21:		Line 21:

	==Notes==		==Notes==
-	* For each entry, headings are written in consecutive order.	+	* For each entry, headings are written in consecutive order of execution.

Mary Mendoza: /* Notes */

Mary Mendoza — Thu, 04 Oct 2012 22:20:31 GMT

Notes

←Older revision		Revision as of 22:20, 4 October 2012
Line 21:		Line 21:

	==Notes==		==Notes==
-	* ~~Place some notes here for visitors~~	+	* For each entry, headings are written in consecutive order.

-	* Example: This project is currently on hold until further notice.