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| ==Objective==
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| * To obtain a spectrum of 40 μM adenosine and inosine
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| * A run of 40 μM adenosine with 50 μM varying inhibitors were conducted using the UV 2500 Shimadzu spectrophotometer.
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| * Three runs of each inhibitor were taken; the average of the runs were taken. The standard deviation were calculated and added as error bars.
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| * Produce histogram
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| ==Procedure for Adenosine and Inosine Spectrum==
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| * To obtain spectrum of 40 μM adenosine, dilute 600 μL of 200 μM adenosine stock solution in 2400 μL 0.05 M phosphate buffer.
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| * Stock solution of 200 μM inosine (MW 268.2 g/mol) was prepared. A spectrum was taken using the same dilution mentioned above.
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| ==Protocol for Assay==
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| * The reagents were added in the following sequence:
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| ** 1.78 mL of phosphate buffer
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| ** 600 μL of 200 μM adenosine (final concentration 50 μM)
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| * This was allowed to mix through venting and placed on the chamber for absorbance reading.
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| ** 300 μL of phosphate buffer (or inhibitor dissolved in phosphate buffer)
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| ** 300 μL of dimethyl sulfoxide (DMSO) (or inhibitor dissolved in DMSO)
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| * The cuvette contents were mixed by venting and placed on the chamber for absorbance reading.
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| ** 20 μL of adenosine deaminase (ADA) was added to catalyze the reaction
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| * The kinetics of the reaction was taken using the UVProbe software.
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| *Cuvette Mixture no Inhibitor
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| [[Image:CuvetteMixtureNoInhibitor.png]]
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| *Cuvette Mixture with Inhibitor
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| [[Image:CuvetteSolnInhibitorHisto.png]]
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| *Inhibitors Ran today:
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| # 50 μM 3-methylacetylsalicylic acid (ZINC 00001221)(Aldrich S376647-1G)
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| # 50 μM Methyl (4-hydroxy-3-methoxyphenyl)acetate (Aldrich PH003466-1MG)
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| # 50 μM [3,4-bis(acetyloxy)benzoic acid] (Aldrich PH00962-150MG)
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