User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/04/17

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Objective)
(Protocol)
Line 8: Line 8:
-
==Protocol==
 
-
* The reagents were added in the following sequence:
 
-
** 1.78 mL of phosphate buffer
 
-
** 600 μL of 200 μM adenosine (final concentration 50 μM)
 
-
* This was allowed to mix through venting and placed on  the chamber for absorbance reading.
 
-
** 300 μL of phosphate buffer (or inhibitor dissolved in phosphate buffer)
 
-
** 300 μL of dimethyl sulfoxide (DMSO) (or inhibitor dissolved in DMSO)
 
-
* The cuvette contents were mixed by venting and placed on the chamber for absorbance reading.
 
-
** 20 μL of adenosine deaminase (ADA) was added to catalyze the reaction
 
-
* The kinetics of the reaction was taken using the UVProbe software.
 
-
*Cuvette Mixture no Inhibitor
 
-
[[Image:CuvetteMixtureNoInhibitor.png]]
 
-
 
-
*Cuvette Mixture with Inhibitor
 
-
[[Image:CuvetteSolnInhibitorHisto.png]]
 
-
 
-
*Inhibitors Ran today:
 
-
#50uM [2,3-bis(actyloxy)benzoic aicd] [PH003446]
 
-
#50uM [Luteolin] [AMB18511398]
 
-
#50uM [Morin] [AMB18511704]
 
-
#50uM [Robinetin] [AMB18511408]
 
-
#50uM [5-Hydroxy-2-methylbenzoic acid] [Aldrich 696366-1G]
 
==Data==
==Data==

Revision as of 17:40, 8 May 2013

Project name Main project page
Previous entry      Next entry



Data


Notes

  • Solution Preparation/Calculations: Catherine Koenigsknecht
  • Cuvette preparation: Mary Mendoza
  • Data Evaluation: Dhea Patel
  • Overall Lab Assistance: Mike Nagle



Personal tools