User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/04/17

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==Protocol==
 
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* The reagents were added in the following sequence:
 
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** 1.78 mL of phosphate buffer
 
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** 600 μL of 200 μM adenosine (final concentration 50 μM)
 
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* This was allowed to mix through venting and placed on  the chamber for absorbance reading.
 
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** 300 μL of phosphate buffer (or inhibitor dissolved in phosphate buffer)
 
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** 300 μL of dimethyl sulfoxide (DMSO) (or inhibitor dissolved in DMSO)
 
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* The cuvette contents were mixed by venting and placed on the chamber for absorbance reading.
 
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** 20 μL of adenosine deaminase (ADA) was added to catalyze the reaction
 
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* The kinetics of the reaction was taken using the UVProbe software.
 
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*Cuvette Mixture no Inhibitor
 
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[[Image:CuvetteMixtureNoInhibitor.png]]
 
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*Cuvette Mixture with Inhibitor
 
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[[Image:CuvetteSolnInhibitorHisto.png]]
 
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*Inhibitors Ran today:
 
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#50uM [2,3-bis(actyloxy)benzoic aicd] [PH003446]
 
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#50uM [Luteolin] [AMB18511398]
 
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#50uM [Morin] [AMB18511704]
 
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#50uM [Robinetin] [AMB18511408]
 
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#50uM [5-Hydroxy-2-methylbenzoic acid] [Aldrich 696366-1G]
 
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==Data==
 
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[[Image:417.png|600px]]
 
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[[Image:410a.png|600px]]
 
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==Notes==
 
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*Solution Preparation/Calculations: Catherine Koenigsknecht
 
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*Cuvette preparation: Mary Mendoza
 
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*Data Evaluation: Dhea Patel
 
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*Overall Lab Assistance: Mike Nagle
 

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