User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/04/16: Difference between revisions

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(Autocreate 2013/04/16 Entry for User:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II)
 
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==Entry title==
==Objective==
* Insert content here...
* A run of 40 μM adenosine with 50 μM varying inhibitors were conducted using the UV 2500 Shimadzu spectrophotometer.
* Three runs of each inhibitor were taken; the average of the runs were taken. The standard deviation were calculated and added as error bars.
* Produce histogram


==Protocol==
* The reagents were added in the following sequence:
** 1.78 mL of phosphate buffer
** 600 μL of 200 μM adenosine (final concentration 50 μM)
* This was allowed to mix through venting and placed on  the chamber for absorbance reading.
** 300 μL of phosphate buffer (or inhibitor dissolved in phosphate buffer)
** 300 μL of dimethyl sulfoxide (DMSO) (or inhibitor dissolved in DMSO)
* The cuvette contents were mixed by venting and placed on the chamber for absorbance reading.
** 20 μL of adenosine deaminase (ADA) was added to catalyze the reaction
* The kinetics of the reaction was taken using the UVProbe software.
*Cuvette Mixture no Inhibitor
[[Image:CuvetteMixtureNoInhibitor.png]]
*Cuvette Mixture with Inhibitor
[[Image:CuvetteSolnInhibitorHisto.png]]
*Inhibitors Ran today:
#4-Acetoxybenozic Acid [ZINC
#3',4',5,5',7-pentahydroxyflavone
#3,6,2',4',5-pentahydroxyflavone
#Datiscetin
==Data==
[[Image:PercentchangeHisot416.png|600px]]
==Notes==
*Solution Preparation/Calculations: Catherine Koenigsknecht
*Cuvette preparation: Mary Mendoza
*Data Evaluation: Dhea Patel
*Overall Lab Assistance: Mike Nagle


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Revision as of 21:11, 18 April 2013

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Objective

  • A run of 40 μM adenosine with 50 μM varying inhibitors were conducted using the UV 2500 Shimadzu spectrophotometer.
  • Three runs of each inhibitor were taken; the average of the runs were taken. The standard deviation were calculated and added as error bars.
  • Produce histogram

Protocol

  • The reagents were added in the following sequence:
    • 1.78 mL of phosphate buffer
    • 600 μL of 200 μM adenosine (final concentration 50 μM)
  • This was allowed to mix through venting and placed on the chamber for absorbance reading.
    • 300 μL of phosphate buffer (or inhibitor dissolved in phosphate buffer)
    • 300 μL of dimethyl sulfoxide (DMSO) (or inhibitor dissolved in DMSO)
  • The cuvette contents were mixed by venting and placed on the chamber for absorbance reading.
    • 20 μL of adenosine deaminase (ADA) was added to catalyze the reaction
  • The kinetics of the reaction was taken using the UVProbe software.
  • Cuvette Mixture no Inhibitor

  • Cuvette Mixture with Inhibitor

  • Inhibitors Ran today:
  1. 4-Acetoxybenozic Acid [ZINC
  2. 3',4',5,5',7-pentahydroxyflavone
  3. 3,6,2',4',5-pentahydroxyflavone
  4. Datiscetin

Data

Notes

  • Solution Preparation/Calculations: Catherine Koenigsknecht
  • Cuvette preparation: Mary Mendoza
  • Data Evaluation: Dhea Patel
  • Overall Lab Assistance: Mike Nagle


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