User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/04/16

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(Objective)
(Protocol)
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==Protocol==
 
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* The reagents were added in the following sequence:
 
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** 1.78 mL of phosphate buffer
 
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** 600 μL of 200 μM adenosine (final concentration 50 μM)
 
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* This was allowed to mix through venting and placed on  the chamber for absorbance reading.
 
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** 300 μL of phosphate buffer (or inhibitor dissolved in phosphate buffer)
 
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** 300 μL of dimethyl sulfoxide (DMSO) (or inhibitor dissolved in DMSO)
 
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* The cuvette contents were mixed by venting and placed on the chamber for absorbance reading.
 
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** 20 μL of adenosine deaminase (ADA) was added to catalyze the reaction
 
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* The kinetics of the reaction was taken using the UVProbe software.
 
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*Cuvette Mixture no Inhibitor
 
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[[Image:CuvetteMixtureNoInhibitor.png]]
 
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*Cuvette Mixture with Inhibitor
 
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[[Image:CuvetteSolnInhibitorHisto.png]]
 
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*Inhibitors Ran today:
 
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#4-Acetoxybenozic Acid [ZINC
 
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#3',4',5,5',7-pentahydroxyflavone
 
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#3,6,2',4',5-pentahydroxyflavone
 
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#Datiscetin
 
==Data==
==Data==

Revision as of 18:38, 8 May 2013

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Data

Notes

  • Solution Preparation/Calculations: Catherine Koenigsknecht
  • Cuvette preparation: Mary Mendoza
  • Data Evaluation: Dhea Patel
  • Overall Lab Assistance: Mike Nagle


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