User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/04/09

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(Autocreate 2013/04/09 Entry for User:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II)
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==Entry title==
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==Objective==
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* Insert content here...
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* Using solutions made last week, a run of 40 μM adenosine with 25 μM varying inhibitors were conducted using the UV 2500 Shimadzu spectrophotometer.
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* Three runs of each inhibitor were taken; the average of the runs were taken. The standard deviation were calculated and added as error bars.
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* Produce histogram
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==Protocol==
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* The reagents were added in the following sequence:
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** 1.78 mL of phosphate buffer
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** 600 μL of 200 μM adenosine (final concentration 50 μM)
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* This was allowed to mix through venting and placed on  the chamber for absorbance reading.
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** 300 μL of phosphate buffer (or inhibitor dissolved in phosphate buffer)
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** 300 μL of dimethyl sulfoxide (DMSO) (or inhibitor dissolved in DMSO)
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* The cuvette contents were mixed by venting and placed on the chamber for absorbance reading.
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** 20 μL of adenosine deaminase (ADA) was added to catalyze the reaction
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* The kinetics of the reaction was taken using the UVProbe software.
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==Description==
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*Cuvette Mixture with Inhibitor
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[[Image:InhibitorCuvetteTable.png]]
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*Cuvette Mixture no Inhibitor
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[[Image:CuvetteMixtureNoInhibitor.png]]
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*Inhibitors Ran today:
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#Aspirin
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#Kaempferol
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==Data==
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* [[Image:409HistogramTable.png]]
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* [[Image:409AverageVelocityHisto.png]]
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* [[Image:409PercentChangeHisto.png]]
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==Notes==
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*Solution Preparation/Calculations: Catherine Koenigsknecht
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*Cuvette preparation: Mary Mendoza
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*Data Evaluation: Dhea Patel
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*Overall Lab Assistance: Mike Nagle
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Revision as of 02:40, 12 April 2013

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Objective

  • Using solutions made last week, a run of 40 μM adenosine with 25 μM varying inhibitors were conducted using the UV 2500 Shimadzu spectrophotometer.
  • Three runs of each inhibitor were taken; the average of the runs were taken. The standard deviation were calculated and added as error bars.
  • Produce histogram

Protocol

  • The reagents were added in the following sequence:
    • 1.78 mL of phosphate buffer
    • 600 μL of 200 μM adenosine (final concentration 50 μM)
  • This was allowed to mix through venting and placed on the chamber for absorbance reading.
    • 300 μL of phosphate buffer (or inhibitor dissolved in phosphate buffer)
    • 300 μL of dimethyl sulfoxide (DMSO) (or inhibitor dissolved in DMSO)
  • The cuvette contents were mixed by venting and placed on the chamber for absorbance reading.
    • 20 μL of adenosine deaminase (ADA) was added to catalyze the reaction
  • The kinetics of the reaction was taken using the UVProbe software.

Description

  • Cuvette Mixture with Inhibitor

Image:InhibitorCuvetteTable.png

  • Cuvette Mixture no Inhibitor

Image:CuvetteMixtureNoInhibitor.png

  • Inhibitors Ran today:
  1. Aspirin
  2. Kaempferol

Data

  • Image:409HistogramTable.png
  • Image:409AverageVelocityHisto.png
  • Image:409PercentChangeHisto.png

Notes

  • Solution Preparation/Calculations: Catherine Koenigsknecht
  • Cuvette preparation: Mary Mendoza
  • Data Evaluation: Dhea Patel
  • Overall Lab Assistance: Mike Nagle


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