User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/04/09

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(Data)
Current revision (17:35, 8 May 2013) (view source)
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==Objective==
 
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* Using solutions made last week, a run of 40 μM adenosine with 50 μM varying inhibitors were conducted using the UV 2500 Shimadzu spectrophotometer.
 
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* Three runs of each inhibitor were taken; the average of the runs were taken. The standard deviation were calculated and added as error bars.
 
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* Produce histogram
 
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==Protocol==
 
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* The reagents were added in the following sequence:
 
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** 1.78 mL of phosphate buffer
 
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** 600 μL of 200 μM adenosine (final concentration 50 μM)
 
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* This was allowed to mix through venting and placed on  the chamber for absorbance reading.
 
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** 300 μL of phosphate buffer (or inhibitor dissolved in phosphate buffer)
 
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** 300 μL of dimethyl sulfoxide (DMSO) (or inhibitor dissolved in DMSO)
 
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* The cuvette contents were mixed by venting and placed on the chamber for absorbance reading.
 
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** 20 μL of adenosine deaminase (ADA) was added to catalyze the reaction
 
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* The kinetics of the reaction was taken using the UVProbe software.
 
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==Description==
 
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*Cuvette Mixture with Inhibitor
 
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[[Image:InhibitorCuvetteTable.png]]
 
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*Cuvette Mixture no Inhibitor
 
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[[Image:CuvetteMixtureNoInhibitor.png]]
 
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*Inhibitors Ran today:
 
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#Aspirin
 
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#Kaempferol
 
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==Data==
 
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* [[Image:409HistogramTable.png|600px]]
 
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* [[Image:409AverageVelocityHisto.png|600px]]
 
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* [[Image:409PercentChangeHisto.png|600px]]
 
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==Notes==
 
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*Solution Preparation/Calculations: Catherine Koenigsknecht
 
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*Cuvette preparation: Mary Mendoza
 
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*Data Evaluation: Dhea Patel
 
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*Overall Lab Assistance: Mike Nagle
 
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