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Preparation of Reagents 500 μM of each inhibitor was made with newly made 0.05 M phosphate buffer solution at the pH of 7.4.
A new stock solution of 200 μM adenosine stock solution was also prepared.
For the kinetic assay runs, it was decided to revert back to the original, chosen adenosine concentration of 40 μM.
Adenosine trials were performed at 265 nm. A histogram of that trial is shown below.
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