User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/04/03: Difference between revisions
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== | ==Preparation of Reagents== | ||
* | * 500 μM of each inhibitor was made with newly made 0.05 M phosphate buffer solution at the pH of 7.4. | ||
* A new stock solution of 200 μM adenosine stock solution was also prepared. | |||
* For the kinetic assay runs, it was decided to revert back to the original, chosen adenosine concentration of 40 μM. | |||
* Adenosine trials were performed at 265 nm. A histogram of that trial is shown below. | |||
[[Image:Pe.png|center]] | |||
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Revision as of 07:17, 5 April 2013
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Preparation of Reagents
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