User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/03/27: Difference between revisions

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* A stock solution of 5-hydroxy-2-methylbenzoic acid was prepared in DMSO.
* A stock solution of 5-hydroxy-2-methylbenzoic acid was prepared in DMSO.
* A 500 μM stock solution of 3-methylacetylsalicylic acid was prepared in 0.05 M phosphate buffer.
* A 500 μM stock solution of 3-methylacetylsalicylic acid was prepared in 0.05 M phosphate buffer.
* Assay for each of the mentioned compounds showed inhibition which can be seen on the data below. The data is provided by [[User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/03/27|Dhea Patel]].


==Preparing 500uM [Inhibitor] Solutions==
*500uM [5-Hydroxy-2-methylbenzoic acid] (Aldrich 696366-1G) [ZINC01668680] made in DMSO:
[[Image:Screen_Shot_2013-03-27_at_1.57.40_PM.png]]
*500uM [3-Methylacetylsalicylic acid] (Aldrich S376647-1G) [ZINC00001221] made in Phosphate Buffer:
[[Image:Screen_Shot_2013-03-27_at_2.49.54_PM.png]]
==Data Table==
[[Image:20130327_Data_Table.tiff]]
* The table above shows the concentrations and volumes of the substrate and other chemical constituents present in the cuvette during data collection.
* The kinetics was performed by the UVProbe of UV 2500 Shimadzu spectrophotometer at 25°C.
==Data==
[[Image:20130327_data.tiff]]
* The histogram above shows that the 4-acetoxybenzoic acid shows the greatest inhibition as an aspirin analog.


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Continuation of Histogram runs

  • The final trial for 4-acetoxybenzoic acid was completed. This compound showed inhibition.
  • A stock solution of 5-hydroxy-2-methylbenzoic acid was prepared in DMSO.
  • A 500 μM stock solution of 3-methylacetylsalicylic acid was prepared in 0.05 M phosphate buffer.
  • Assay for each of the mentioned compounds showed inhibition which can be seen on the data below. The data is provided by Dhea Patel.

Preparing 500uM [Inhibitor] Solutions

  • 500uM [5-Hydroxy-2-methylbenzoic acid] (Aldrich 696366-1G) [ZINC01668680] made in DMSO:

  • 500uM [3-Methylacetylsalicylic acid] (Aldrich S376647-1G) [ZINC00001221] made in Phosphate Buffer:

Data Table

  • The table above shows the concentrations and volumes of the substrate and other chemical constituents present in the cuvette during data collection.
  • The kinetics was performed by the UVProbe of UV 2500 Shimadzu spectrophotometer at 25°C.

Data

  • The histogram above shows that the 4-acetoxybenzoic acid shows the greatest inhibition as an aspirin analog.


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