User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/03/27

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(Continuation of Histogram runs)
Current revision (18:29, 8 May 2013) (view source)
(Data)
 
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==Continuation of Histogram runs==
 
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* The final trial for 4-acetoxybenzoic acid was completed. This compound showed inhibition.
 
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* A stock solution of 5-hydroxy-2-methylbenzoic acid was prepared in DMSO.
 
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* A 500 μM stock solution of 3-methylacetylsalicylic acid was prepared in 0.05 M phosphate buffer.
 
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* Assay for each of the mentioned compounds showed inhibition which can be seen on the data below. The data is provided by [[User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/03/27|Dhea Patel]].
 
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==Preparing 500uM [Inhibitor] Solutions==
 
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*500uM [5-Hydroxy-2-methylbenzoic acid] (Aldrich 696366-1G) [ZINC01668680] made in DMSO:
 
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[[Image:Screen_Shot_2013-03-27_at_1.57.40_PM.png]]
 
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*500uM [3-Methylacetylsalicylic acid] (Aldrich S376647-1G) [ZINC00001221] made in Phosphate Buffer:
 
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[[Image:Screen_Shot_2013-03-27_at_2.49.54_PM.png]]
 
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==Data Table==
 
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[[Image:20130327_Data_Table.tiff]]
 
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* The table above shows the concentrations and volumes of the substrate and other chemical constituents present in the cuvette during data collection.
 
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* The kinetics was performed by the UVProbe of UV 2500 Shimadzu spectrophotometer at 25°C.
 
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==Data==
 
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[[Image:20130327_data.tiff]]
 
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* The histogram above shows that the 4-acetoxybenzoic acid shows the greatest inhibition as an aspirin analog.
 
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