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Preparing 500uM [Inhibitor] Solutions
- 500uM [5-Hydroxy-2-methylbenzoic acid] (Aldrich 696366-1G) [ZINC01668680] made in DMSO:
- 500uM [3-Methylacetylsalicylic acid] (Aldrich S376647-1G) [ZINC00001221] made in Phosphate Buffer:
Data Table
- The table above shows the concentrations and volumes of the substrate and other chemical constituents present in the cuvette during data collection.
- The kinetics was performed by the UVProbe of UV 2500 Shimadzu spectrophotometer at 25°C.
Data
- The histogram above shows that the 4-acetoxybenzoic acid shows the greatest inhibition as an aspirin analog.
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