User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/03/26: Difference between revisions
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== | ==25 uM Adenosine Histogram Kinetic Assay Runs== | ||
* | * The following procedures and data were taken from [[User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/03/26|Dhea Patel]]. | ||
==Preparing 500uM Acetylsalicylic Acid Stock Solution== | |||
*13.7mg acetylsalicylic acid was added to 506.957mL phosphate buffer: | |||
[[Image:Screen_Shot_2013-03-26_at_12.39.06_PM.png]] | |||
==Preparing 500uM 4-Acetoxybenzoic acid (Aldrich 248517-50G) Stock Solution== | |||
*4-Acetoxybenzoic acid (Aldrich 248517-50G) (180.17g/mol) [ZINC00225517]: | |||
*2.2mg 4-Acetoxybenzoic acid (Aldrich 248517-50G) was added to 24.4214mL phosphate buffer. | |||
*[[Image:Screen_Shot_2013-03-26_at_3.11.27_PM.png]] | |||
==Kinetics== | |||
*Data Table of Volumes and Concentrations Used | |||
[[Image:20130326_Data_Table.tiff|px200]] | |||
==Data== | |||
[[Image:20130326_Data_.tiff]] | |||
*The aspirin appears to be aiding the reaction rather than inhibiting the adenosine. | |||
Revision as of 13:24, 28 March 2013
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25 uM Adenosine Histogram Kinetic Assay Runs
Preparing 500uM Acetylsalicylic Acid Stock Solution
Preparing 500uM 4-Acetoxybenzoic acid (Aldrich 248517-50G) Stock Solution
Kinetics
Data
|