User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/03/26

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(25 uM Adenosine Histogram Kinetic Assay Runs)
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==25 uM Adenosine Histogram Kinetic Assay Runs==
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* The following procedures and data were taken from [[User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/03/26|Dhea Patel]].
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==Preparing 500uM Acetylsalicylic Acid Stock Solution==
==Preparing 500uM Acetylsalicylic Acid Stock Solution==

Revision as of 17:26, 8 May 2013

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Preparing 500uM Acetylsalicylic Acid Stock Solution

  • 13.7mg acetylsalicylic acid was added to 506.957mL phosphate buffer:

Image:Screen_Shot_2013-03-26_at_12.39.06_PM.png


Preparing 500uM 4-Acetoxybenzoic acid (Aldrich 248517-50G) Stock Solution

  • 4-Acetoxybenzoic acid (Aldrich 248517-50G) (180.17g/mol) [ZINC00225517]:
  • 2.2mg 4-Acetoxybenzoic acid (Aldrich 248517-50G) was added to 24.4214mL phosphate buffer.
  • Image:Screen_Shot_2013-03-26_at_3.11.27_PM.png

Kinetics

  • Data Table of Volumes and Concentrations Used

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Data

Image:20130326_Data_.tiff

  • The aspirin appears to be aiding the reaction rather than inhibiting the adenosine.



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