User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/03/20: Difference between revisions
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== | ==Histogram== | ||
* | * It was decided to create a histogram for each inhibitor which includes the flavonoids and the non-nucleoside, acetylsalicylic acid analogs. Each inhibitor consisted of three trials. | ||
* From Sigma, 500 μM Kaempferol (C<sub>15</sub>H<sub>10</sub>O<sub>6</sub>, MW 286.24 g/mol) stock solution was prepared in dimethylsulfoxide (DMSO). | |||
* A kinetic assay run of 40 μM adenosine without inhibitors was executed through the kinetics of the UVProbe system. | |||
* After the three trials of 40 μM adenosine, aspirin trials were conducted. | |||
* The aspirin trials did not show inhibition. A possible conclusion for the inconsistency was that the aspirin stock solution has deteriorated. | |||
* Kaempferol trials were started. | |||
* After examining some of the data, it was concluded to adjust the 40 μM adenosine concentration to 25 μM. In addition, cuvettes will also contain 300 μL of DMSO. | |||
* For the following week, a new aspiring stock solution will be prepared. | |||
==adenosine vs. aspirin== | |||
* The histogram below displays the non-inhibitory effect of aspirin. | |||
[[Image:Bar2.png]] | |||
==Tables== | |||
* The data below was taken from [[User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/03/20|Dhea Patel]]. | |||
*To measure the mass of Kaempferol and the volume of DMSO: | |||
[[Image:Screen_Shot_2013-03-20_at_1.45.50_PM.png]] | |||
*Table of volumes and concentrations: | |||
[[Image:Kinetics_Histogram_table.tiff|px550]] | |||
Revision as of 17:56, 22 March 2013
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Histogram
adenosine vs. aspirin
Tables
|