User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/03/20

(Difference between revisions)

Revision as of 13:13, 22 March 2013

Project name

It was decided to create a histogram for each inhibitor which includes the flavonoids and the non-nucleoside, acetylsalicylic acid analogs. Each inhibitor consisted of three trials.
From Sigma, 500 μM Kaempferol (C₁₅H₁₀O₆, MW 286.24 g/mol) stock solution was prepared in dimethylsulfoxide (DMSO).
A kinetic assay run of 40 μM adenosine without inhibitors was executed through the kinetics of the UVProbe system.
After the three trials of 40 μM adenosine, aspirin trials were conducted.
The aspirin trials did not show inhibition. A possible conclusion for the inconsistency was that the aspirin stock solution has deteriorated.
Kaempferol trials were started.
After examining some of the data, it was concluded to adjust the 40 μM adenosine concentration to 25 μM.

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 * The aspirin trials did not show inhibition. A possible conclusion for the inconsistency was that the aspirin stock solution has deteriorated.
 * Kaempferol trials were started.
+* After examining some of the data, it was concluded to adjust the 40 μM adenosine concentration to 25 μM.
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