User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/03/19

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75 μM Aspirin inhibitor

  • The following procedures were taken from Dhea Patel.
  • Inhibitor kinetics using 75uM acetylsalicylic acid was performed.
  • Adenosine stock solutions were prepared using the following table:

Image:Screen_Shot_2013-03-19_at_1.25.12_PM.png

  • The volumes and concentrations added to each cuvette is outlined in the following table:

Image:Screen_Shot_2013-03-19_at_1.25.03_PM.png

Data

Image:75uM_inhibitor.tiff

The Km value was lower than anticipated.


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