User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/27: Difference between revisions
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==ADA | |||
* | *Taken from [[User:Dhea_Patel/Notebook/CHEM_572:_ADA%26Inhibitor_Kinetics/2013/02/27|Dhea Patel's Notebook]]. Everything was done together. | ||
==Objective== | |||
*to begin ADA + Inhibitor kinetics | |||
==Description== | |||
*150uM Acetylsalicylic Acid Inhibitor stock solution (in Phosphate buffer) was made using the following calculations: | |||
[[Image:Screen_Shot_2013-02-27_at_12.43.29_PM.png]] | |||
*25 and 40uM with [Inhibitor] = 0 was re-run for Trial 3. | |||
*More 0.05M phosphate buffer was prepared according to the procedure outlined in [[User:Dhea_Patel/Notebook/CHEM_572:_ADA%26Inhibitor_Kinetics/2013/01/23| January's entry]]. | |||
*ADA kinetics in the presence of the Acetylsalicylic Acid was started using the following table: | |||
[[Image:Screen_Shot_2013-02-27_at_2.57.04_PM.png]] | |||
==Data== | |||
[[Image:20130227_ADA_Kinetics_3T.tiff|px200]] | |||
[[Image:20130227_Lineweaver_Burk_ADA_Kinetics_3T.tiff|px200]] | |||
[[Image:20130227_average_ADA_Kinetics_3T.tiff|px200]] | |||
Revision as of 13:06, 27 February 2013
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Objective
Description
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