User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/19: Difference between revisions

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==ADA Kinetic Assay with Acetylsalicylic Acid inhibitor==
* The objective for this laboratory period was to run ADA Kinetic assay with the addition of the inhibitor.
* Acetylsalicylic acid (C<sub>9</sub>H<sub>8</sub>O<sub>4</sub>, MW 180.16) was obtained from Sigma-Aldrich, Co.
* The assay of the acid is ≥ 99.0 %. The solid appeared as a white crystalline form having an absorbance of 230 nm.




* The first trial conducted did not include the inhibitor for the purpose of obtaining a zero point.
* There were observed inconsistencies among the 32, 39.7, 141.8, and 200 μM adenosine concentrations. Thus, new concentrations were calculated for adenosine.


==Protocol==
* The portion below was taken from the note book of [[User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/02/19
|Dhea Patel]].
* [[Image:Screen_Shot_2013-02-19_at_1.12.45_PM.png]]
[[Image:Screen_Shot_2013-02-19_at_2.46.05_PM.png]]
*The data obtained from the ADA Assay was not consistent with previous findings, so new Adenosine concentrations were made up according to the following table:
[[Image:Screen_Shot_2013-02-19_at_3.19.14_PM.png]]
==Data==
[[Image:NOInhibitorAssay.tiff]]




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