User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/13 - Revision history

User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/13 - Revision history http://openwetware.org/index.php?title=User:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II/2013/02/13&action=history Revision history for this page on the wiki en MediaWiki 1.13.2 Thu, 23 May 2013 08:44:22 GMT Mary Mendoza: /* Data */ http://openwetware.org/index.php?title=User:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II/2013/02/13&diff=697275&oldid=prev <p><span class="autocomment">Data</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 22:16, 8 May 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 8:</td> <td colspan="2" class="diff-lineno">Line 8:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">==Data==</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[Image:Average_Lineweaver-Burk_Plot.png]]</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[Image:Average_Velocities.png]]</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[Image:Screen_Shot_2013-02-19_at_11.14.56_AM.png]]</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>  </table> Wed, 08 May 2013 22:16:55 GMT Mary Mendoza http://openwetware.org/wiki/User_talk:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II/2013/02/13 Mary Mendoza: /* Trial 2 & 3 of ADA Kinetic Assay */ http://openwetware.org/index.php?title=User:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II/2013/02/13&diff=697274&oldid=prev <p><span class="autocomment">Trial 2 & 3 of ADA Kinetic Assay</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 22:16, 8 May 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 6:</td> <td colspan="2" class="diff-lineno">Line 6:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>| colspan="2"|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>| colspan="2"|</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">==Trial 2 & 3 of ADA Kinetic Assay==</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* The agenda for this laboratory period are the completion of trials 2 and 3 of ADA Kinetic Assay and obtain the K<sub>m</sub> and V<sub>max</sub> of the enzymatic reaction.</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* UV2550 Shimadzu Spectrophotometer was baseline with phosphate buffer. The temperature control of the CPS controller was set at 25°C.</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* Baseline was performed by preparing two cuvettes filled with phosphate buffer, one placed on the blank slot and the other at cell slot 1.</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* After baseline, an assay containing 150 μM adenosine was collected using the UV Probe. Trial 2 was complete by running an assay with the 200 μM adenosine.</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* The concentration of each sample was obtained from  the calculations below:</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[Image:Data_Table.JPG|center]]</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* During the previous laboratory data collection of 100 μM trial 2, an error was encountered by the opening of the cell chamber. Thus, it was decided to repeat the run for 100 μM. </del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* Ran kinetics on 2.56 uM solution (20uL ADA, 600uL 12.8 uM adenosine, and 2.38 mL pH7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">** dAbs was obtained and plotted vs. time in excel.</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* Ran kinetics on 6.4 uM solution (20uL ADA, 600uL 32 uM adenosine, and 2.38 mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">** dAbs was obtained and plotted vs. time in excel.</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* Ran kinetics on 16 uM solution (20uL ADA, 600uL 80 uM adenosine, and 2.38 mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">** dAbs was obtained and plotted vs. time in excel.</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* Ran kinetics on 40 uM solution (20uL ADA, 600uL 200 uM adenosine, and 2.38 mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">** dAbs was obtained and plotted vs. time in excel.</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* Ran kinetics on 100 uM solution (20uL ADA, 600uL 500 uM adenosine, and 2.3 8mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">** dAbs was obtained and plotted vs. time in excel.</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* Ran kinetics on 150 uM solution (20uL ADA, 600uL 750 uM adenosine, and 2.3 8mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">** dAbs was obtained and plotted vs. time in excel.</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* Ran kinetics on 200 uM solution (20uL ADA, 600uL 1000 uM adenosine, and 2.3 8mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">** dAbs was obtained and plotted vs. time in excel.</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* Average velocities were obtained.</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* The completion of trials 2 & 3 were followed by the calculation for the K<sub>m</sub> and V<sub>max</sub>. Data was exchanged with Catherine Koenigsknecht and Michael Nagle.</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* The excel worksheet of the finalized data is attached below.</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[http://openwetware.org/wiki/Image:20130213_Final_ADA_kinetics_Data.xlsx ADA Kinetics Assay]</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Data==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Data==</div></td></tr>  </table> Wed, 08 May 2013 22:16:44 GMT Mary Mendoza http://openwetware.org/wiki/User_talk:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II/2013/02/13 Mary Mendoza: /* Trial 2 & 3 of ADA Kinetic Assay */ http://openwetware.org/index.php?title=User:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II/2013/02/13&diff=677998&oldid=prev <p><span class="autocomment">Trial 2 & 3 of ADA Kinetic Assay</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 15:03, 20 February 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 38:</td> <td colspan="2" class="diff-lineno">Line 38:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[http://openwetware.org/wiki/Image:20130213_Final_ADA_kinetics_Data.xlsx ADA Kinetics Assay]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[http://openwetware.org/wiki/Image:20130213_Final_ADA_kinetics_Data.xlsx ADA Kinetics Assay]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==Data==</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:Average_Lineweaver-Burk_Plot.png]]</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:Average_Velocities.png]]</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:Screen_Shot_2013-02-19_at_11.14.56_AM.png]]</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>  </table> Wed, 20 Feb 2013 15:03:19 GMT Mary Mendoza http://openwetware.org/wiki/User_talk:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II/2013/02/13 Mary Mendoza: /* Trial 2 & 3 of ADA Kinetic Assay */ http://openwetware.org/index.php?title=User:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II/2013/02/13&diff=676697&oldid=prev <p><span class="autocomment">Trial 2 & 3 of ADA Kinetic Assay</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 15:19, 15 February 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 36:</td> <td colspan="2" class="diff-lineno">Line 36:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* The excel worksheet of the finalized data is attached below.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* The excel worksheet of the finalized data is attached below.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[http://openwetware.org/wiki/Image:20130213_Final_ADA_kinetics_Data.xlsx<del class="diffchange diffchange-inline">|</del>ADA Kinetics Assay]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[http://openwetware.org/wiki/Image:20130213_Final_ADA_kinetics_Data.xlsx ADA Kinetics Assay]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>  </table> Fri, 15 Feb 2013 15:19:09 GMT Mary Mendoza http://openwetware.org/wiki/User_talk:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II/2013/02/13 Mary Mendoza: /* Trial 2 & 3 of ADA Kinetic Assay */ http://openwetware.org/index.php?title=User:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II/2013/02/13&diff=676696&oldid=prev <p><span class="autocomment">Trial 2 & 3 of ADA Kinetic Assay</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 15:17, 15 February 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 36:</td> <td colspan="2" class="diff-lineno">Line 36:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* The excel worksheet of the finalized data is attached below.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* The excel worksheet of the finalized data is attached below.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[<del class="diffchange diffchange-inline">[</del>Image:<del class="diffchange diffchange-inline">20130213 Final ADA kinetics Data</del>.xlsx|ADA Kinetics Assay <del class="diffchange diffchange-inline">Data]</del>]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[<ins class="diffchange diffchange-inline">http://openwetware.org/wiki/</ins>Image:<ins class="diffchange diffchange-inline">20130213_Final_ADA_kinetics_Data</ins>.xlsx|ADA Kinetics Assay]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>  </table> Fri, 15 Feb 2013 15:17:18 GMT Mary Mendoza http://openwetware.org/wiki/User_talk:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II/2013/02/13 Mary Mendoza: /* Trial 2 & 3 of ADA Kinetic Assay */ http://openwetware.org/index.php?title=User:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II/2013/02/13&diff=676694&oldid=prev <p><span class="autocomment">Trial 2 & 3 of ADA Kinetic Assay</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 15:15, 15 February 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 36:</td> <td colspan="2" class="diff-lineno">Line 36:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* The excel worksheet of the finalized data is attached below.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* The excel worksheet of the finalized data is attached below.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:20130213 Final ADA kinetics Data.xlsx]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:20130213 Final ADA kinetics Data.xlsx<ins class="diffchange diffchange-inline">|ADA Kinetics Assay Data</ins>]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>  </table> Fri, 15 Feb 2013 15:15:56 GMT Mary Mendoza http://openwetware.org/wiki/User_talk:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II/2013/02/13 Mary Mendoza: /* Trial 2 & 3 of ADA Kinetic Assay */ http://openwetware.org/index.php?title=User:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II/2013/02/13&diff=676693&oldid=prev <p><span class="autocomment">Trial 2 & 3 of ADA Kinetic Assay</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 15:15, 15 February 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 33:</td> <td colspan="2" class="diff-lineno">Line 33:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** dAbs was obtained and plotted vs. time in excel.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** dAbs was obtained and plotted vs. time in excel.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Average velocities were obtained.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Average velocities were obtained.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* The completion of trials 2 & 3 were followed by the calculation for the K<sub>m</sub> and V<sub>max</sub. Data was exchanged with Catherine Koenigsknecht and Michael Nagle.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* The completion of trials 2 & 3 were followed by the calculation for the K<sub>m</sub> and V<sub>max</sub<ins class="diffchange diffchange-inline">></ins>. Data was exchanged with Catherine Koenigsknecht and Michael Nagle.</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* The excel worksheet of the finalized data is attached below.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[Image:20130213 Final ADA kinetics Data.xlsx]]</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>  </table> Fri, 15 Feb 2013 15:15:25 GMT Mary Mendoza http://openwetware.org/wiki/User_talk:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II/2013/02/13 Mary Mendoza: /* Trial 2 & 3 of ADA Kinetic Assay */ http://openwetware.org/index.php?title=User:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II/2013/02/13&diff=676129&oldid=prev <p><span class="autocomment">Trial 2 & 3 of ADA Kinetic Assay</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:05, 13 February 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 30:</td> <td colspan="2" class="diff-lineno">Line 30:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Ran kinetics on 150 uM solution (20uL ADA, 600uL 750 uM adenosine, and 2.3 8mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Ran kinetics on 150 uM solution (20uL ADA, 600uL 750 uM adenosine, and 2.3 8mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** dAbs was obtained and plotted vs. time in excel.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** dAbs was obtained and plotted vs. time in excel.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Ran kinetics on 200 uM solution (20uL ADA, 600uL 1000 uM adenosine, and 2.3 8mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Ran kinetics on 200 uM solution (20uL ADA, 600uL 1000 uM adenosine, and 2.3 8mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** dAbs was obtained and plotted vs. time in excel.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** dAbs was obtained and plotted vs. time in excel.</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* Average velocities were obtained.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* The completion of trials 2 & 3 were followed by the calculation for the K<sub>m</sub> and V<sub>max</sub. Data was exchanged with Catherine Koenigsknecht and Michael Nagle.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* Outliers were observed when the trials were graphed. These outliers were from concentrations 6.4 μM and 200 μM. Therefore, it was decided to re-perform the assay for these two concentrations.</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>  </table> Wed, 13 Feb 2013 20:05:26 GMT Mary Mendoza http://openwetware.org/wiki/User_talk:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II/2013/02/13 Mary Mendoza: /* Trial 2 & 3 of ADA Kinetic Assay */ http://openwetware.org/index.php?title=User:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II/2013/02/13&diff=676114&oldid=prev <p><span class="autocomment">Trial 2 & 3 of ADA Kinetic Assay</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 19:11, 13 February 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 26:</td> <td colspan="2" class="diff-lineno">Line 26:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Ran kinetics on 40 uM solution (20uL ADA, 600uL 200 uM adenosine, and 2.38 mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Ran kinetics on 40 uM solution (20uL ADA, 600uL 200 uM adenosine, and 2.38 mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** dAbs was obtained and plotted vs. time in excel.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** dAbs was obtained and plotted vs. time in excel.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Ran kinetics on 100 uM solution (20uL ADA, 600uL 500 uM adenosine, and 2.3 8mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Ran kinetics on 100 uM solution (20uL ADA, 600uL 500 uM adenosine, and 2.3 8mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** dAbs was obtained and plotted vs. time in excel.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** dAbs was obtained and plotted vs. time in excel.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Ran kinetics on 150 uM solution (20uL ADA, 600uL 750 uM adenosine, and 2.3 8mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Ran kinetics on 150 uM solution (20uL ADA, 600uL 750 uM adenosine, and 2.3 8mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** dAbs was obtained and plotted vs. time in excel.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** dAbs was obtained and plotted vs. time in excel.</div></td></tr> <tr><td colspan="2" class="diff-lineno">Line 35:</td> <td colspan="2" class="diff-lineno">Line 33:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Ran kinetics on 200 uM solution (20uL ADA, 600uL 1000 uM adenosine, and 2.3 8mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Ran kinetics on 200 uM solution (20uL ADA, 600uL 1000 uM adenosine, and 2.3 8mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** dAbs was obtained and plotted vs. time in excel.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>** dAbs was obtained and plotted vs. time in excel.</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* Outliers were observed when the trials were graphed. These outliers were from concentrations 6.4 μM and 200 μM. Therefore, it was decided to re-perform the assay for these two concentrations.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>  </table> Wed, 13 Feb 2013 19:11:54 GMT Mary Mendoza http://openwetware.org/wiki/User_talk:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II/2013/02/13 Mary Mendoza: /* Trial 2 & 3 of ADA Kinetic Assay */ http://openwetware.org/index.php?title=User:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II/2013/02/13&diff=676102&oldid=prev <p><span class="autocomment">Trial 2 & 3 of ADA Kinetic Assay</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 18:52, 13 February 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 18:</td> <td colspan="2" class="diff-lineno">Line 18:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* During the previous laboratory data collection of 100 μM trial 2, an error was encountered by the opening of the cell chamber. Thus, it was decided to repeat the run for 100 μM.  </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* During the previous laboratory data collection of 100 μM trial 2, an error was encountered by the opening of the cell chamber. Thus, it was decided to repeat the run for 100 μM.  </div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* Ran kinetics on 2.<del class="diffchange diffchange-inline">56uM </del>solution (20uL ADA, 600uL 12.<del class="diffchange diffchange-inline">8uM Adenosine</del>, and 2.<del class="diffchange diffchange-inline">38mL </del>pH7.4, 0.<del class="diffchange diffchange-inline">005M </del>phosphate buffer) for 5 minutes at <del class="diffchange diffchange-inline">265nm</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* Ran kinetics on 2.<ins class="diffchange diffchange-inline">56 uM </ins>solution (20uL ADA, 600uL 12.<ins class="diffchange diffchange-inline">8 uM adenosine</ins>, and 2.<ins class="diffchange diffchange-inline">38 mL </ins>pH7.4, 0.<ins class="diffchange diffchange-inline">05M </ins>phosphate buffer) for 5 minutes at <ins class="diffchange diffchange-inline">265 nm</ins>.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>** dAbs was obtained and plotted vs. time in excel</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>** dAbs was obtained and plotted vs. time in excel<ins class="diffchange diffchange-inline">.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* Ran kinetics on 6.4 uM solution (20uL ADA, 600uL 32 uM adenosine, and 2.38 mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">** dAbs was obtained and plotted vs. time in excel.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* Ran kinetics on 16 uM solution (20uL ADA, 600uL 80 uM adenosine, and 2.38 mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">** dAbs was obtained and plotted vs. time in excel.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* Ran kinetics on 40 uM solution (20uL ADA, 600uL 200 uM adenosine, and 2.38 mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">** dAbs was obtained and plotted vs. time in excel.</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* Ran kinetics on 100 uM solution (20uL ADA, 600uL 500 uM adenosine, and 2.3 8mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">** dAbs was obtained and plotted vs. time in excel.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* Ran kinetics on 150 uM solution (20uL ADA, 600uL 750 uM adenosine, and 2.3 8mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">** dAbs was obtained and plotted vs. time in excel.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* Ran kinetics on 200 uM solution (20uL ADA, 600uL 1000 uM adenosine, and 2.3 8mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">** dAbs was obtained and plotted vs. time in excel.</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>  </table> Wed, 13 Feb 2013 18:52:31 GMT Mary Mendoza http://openwetware.org/wiki/User_talk:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II/2013/02/13