User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/13: Difference between revisions
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* During the previous laboratory data collection of 100 μM trial 2, an error was encountered by the opening of the cell chamber. Thus, it was decided to repeat the run for 100 μM. | * During the previous laboratory data collection of 100 μM trial 2, an error was encountered by the opening of the cell chamber. Thus, it was decided to repeat the run for 100 μM. | ||
* Ran kinetics on 2. | * Ran kinetics on 2.56 uM solution (20uL ADA, 600uL 12.8 uM adenosine, and 2.38 mL pH7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm. | ||
** dAbs was obtained and plotted vs. time in excel | ** dAbs was obtained and plotted vs. time in excel. | ||
* Ran kinetics on 6.4 uM solution (20uL ADA, 600uL 32 uM adenosine, and 2.38 mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm. | |||
** dAbs was obtained and plotted vs. time in excel. | |||
* Ran kinetics on 16 uM solution (20uL ADA, 600uL 80 uM adenosine, and 2.38 mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm. | |||
** dAbs was obtained and plotted vs. time in excel. | |||
* Ran kinetics on 40 uM solution (20uL ADA, 600uL 200 uM adenosine, and 2.38 mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm. | |||
** dAbs was obtained and plotted vs. time in excel. | |||
* Ran kinetics on 100 uM solution (20uL ADA, 600uL 500 uM adenosine, and 2.3 8mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm. | |||
** dAbs was obtained and plotted vs. time in excel. | |||
* Ran kinetics on 150 uM solution (20uL ADA, 600uL 750 uM adenosine, and 2.3 8mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm. | |||
** dAbs was obtained and plotted vs. time in excel. | |||
* Ran kinetics on 200 uM solution (20uL ADA, 600uL 1000 uM adenosine, and 2.3 8mL pH 7.4, 0.05M phosphate buffer) for 5 minutes at 265 nm. | |||
** dAbs was obtained and plotted vs. time in excel. | |||
Revision as of 11:52, 13 February 2013
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Trial 2 & 3 of ADA Kinetic Assay
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