User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/13

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Trial 2 & 3 of ADA Kinetic Assay)
(Trial 2 & 3 of ADA Kinetic Assay)
Line 9: Line 9:
* UV2550 Shimadzu Spectrophotometer was baseline with phosphate buffer. The temperature control of the CPS controller was set at 25°C.
* UV2550 Shimadzu Spectrophotometer was baseline with phosphate buffer. The temperature control of the CPS controller was set at 25°C.
* Baseline was performed by preparing two cuvettes filled with phosphate buffer, one placed on the blank slot and the other at cell slot 1.
* Baseline was performed by preparing two cuvettes filled with phosphate buffer, one placed on the blank slot and the other at cell slot 1.
-
* After baseline, an assay containing 150 μM adenosine was collected using the UV Probe.
+
* After baseline, an assay containing 150 μM adenosine was collected using the UV Probe. Trial 2 was complete by running an assay with the 200 μM adenosine.
 +
* The concentration of each sample was obtained from  the calculations below:
 +
 
 +
[[Image:Data_Table.JPG|center]]

Revision as of 13:42, 13 February 2013

Project name Main project page
Previous entry      Next entry

Trial 2 & 3 of ADA Kinetic Assay

  • UV2550 Shimadzu Spectrophotometer was baseline with phosphate buffer. The temperature control of the CPS controller was set at 25°C.
  • Baseline was performed by preparing two cuvettes filled with phosphate buffer, one placed on the blank slot and the other at cell slot 1.
  • After baseline, an assay containing 150 μM adenosine was collected using the UV Probe. Trial 2 was complete by running an assay with the 200 μM adenosine.
  • The concentration of each sample was obtained from the calculations below:



Personal tools