User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/13

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(Trial 2 & 3 of ADA Kinetic Assay)
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==Trial 2 & 3 of ADA Kinetic Assay==
 
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* UV2550 Shimadzu Spectrophotometer was baseline with phosphate buffer. The temperature control of the CPS controller was set at 25°C.
 
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* Baseline was performed by preparing two cuvettes filled with phosphate buffer, one placed on the blank slot and the other at cell slot 1.
 
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* After baseline, an assay containing 150 μM adenosine was collected using the UV Probe. Trial 2 was complete by running an assay with the 200 μM adenosine.
 
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* The concentration of each sample was obtained from  the calculations below:
 
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[[Image:Data_Table.JPG|center]]
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