User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/13: Difference between revisions
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==Trial 2 & 3 of ADA Kinetic Assay== | ==Trial 2 & 3 of ADA Kinetic Assay== | ||
* The agenda for this laboratory period are the completion of trials 2 and 3 of ADA Kinetic Assay and obtain the K<sub>m</sub> and V<sub>max</sub> of the enzymatic reaction. | |||
* UV2550 Shimadzu Spectrophotometer was baseline with phosphate buffer. The temperature control of the CPS controller was set at 25°C. | * UV2550 Shimadzu Spectrophotometer was baseline with phosphate buffer. The temperature control of the CPS controller was set at 25°C. | ||
* Baseline was performed by preparing two cuvettes filled with phosphate buffer, one placed on the blank slot and the other at cell slot 1. | * Baseline was performed by preparing two cuvettes filled with phosphate buffer, one placed on the blank slot and the other at cell slot 1. | ||
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[[Image:Data_Table.JPG|center]] | [[Image:Data_Table.JPG|center]] | ||
* During the previous laboratory data collection of 100 μM trial 2, an error was encountered by the opening of the cell chamber. Thus, it was decided to repeat the run for 100 μM. | |||
Revision as of 10:53, 13 February 2013
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Trial 2 & 3 of ADA Kinetic Assay
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