User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/13: Difference between revisions
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* UV2550 Shimadzu Spectrophotometer was baseline with phosphate buffer. The temperature control of the CPS controller was set at 25°C. | * UV2550 Shimadzu Spectrophotometer was baseline with phosphate buffer. The temperature control of the CPS controller was set at 25°C. | ||
* Baseline was performed by preparing two cuvettes filled with phosphate buffer, one placed on the blank slot and the other at cell slot 1. | * Baseline was performed by preparing two cuvettes filled with phosphate buffer, one placed on the blank slot and the other at cell slot 1. | ||
* After baseline, an assay containing 150 μM adenosine was collected using the UV Probe. | * After baseline, an assay containing 150 μM adenosine was collected using the UV Probe. Trial 2 was complete by running an assay with the 200 μM adenosine. | ||
* The concentration of each sample was obtained from the calculations below: | |||
[[Image:Data_Table.JPG|center]] | |||
Revision as of 10:42, 13 February 2013
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Trial 2 & 3 of ADA Kinetic Assay
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