User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/02/13

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(Trial 2 & 3 of ADA Kinetic Assay)
(Trial 2 & 3 of ADA Kinetic Assay)
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* UV2550 Shimadzu Spectrophotometer was baseline with phosphate buffer. The temperature control of the CPS controller was set at 25°C.
* UV2550 Shimadzu Spectrophotometer was baseline with phosphate buffer. The temperature control of the CPS controller was set at 25°C.
* Baseline was performed by preparing two cuvettes filled with phosphate buffer, one placed on the blank slot and the other at cell slot 1.
* Baseline was performed by preparing two cuvettes filled with phosphate buffer, one placed on the blank slot and the other at cell slot 1.
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* After baseline,  
+
* After baseline, an assay containing 150 μM adenosine was collected using the UV Probe.

Revision as of 13:39, 13 February 2013

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Trial 2 & 3 of ADA Kinetic Assay

  • UV2550 Shimadzu Spectrophotometer was baseline with phosphate buffer. The temperature control of the CPS controller was set at 25°C.
  • Baseline was performed by preparing two cuvettes filled with phosphate buffer, one placed on the blank slot and the other at cell slot 1.
  • After baseline, an assay containing 150 μM adenosine was collected using the UV Probe.



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