User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/01/29 - Revision history

Mary Mendoza: /* Preparation of ADA Kinetic Assay Reactants */

2013-05-08T22:10:47Z

Preparation of ADA Kinetic Assay Reactants

←Older revision		Revision as of 22:10, 8 May 2013
Line 6:		Line 6:
	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	~~==Preparation of ADA Kinetic Assay Reactants==~~
-	* The agenda is to prepare ADA Kinetic Assay reagents for next week's Kinetic Assay runs.
-	* Adenosine deaminase was ordered from Worthington (19.8 u/mg). To obtain 0.5 M per mL, the calculation below was made amounting to 0.0253.
-
-
-	~~<math>\frac{0.5 units}{19.8 u/mg}</math> = 0.0253 mg of ADA in 1 mL phosphate buffer~~
-
-
-	* For convenience, it was decided to dissolve 2.5 mg into 100 mL of phosphate buffer. The final amount weighed 2.0 mg which will be dissolved into 80 mL of phosphate buffer. ADA was refrigerated between 2-8°C temporarily.
-
-	* In preparation of the sodium phosphate buffer, 3.1093 g of sodium phosphate monobasic (chempure, FW 268.07 g/mol) and 10.9019 g of sodium phosphate dibasic (fisher scientific, FW 137.99 g/mol) were dissolved in 1 L water to obtain a 0.1 M sodium phosphate buffer solution.
-	* The pH of the buffer was adjusted to 7.4 by the addition of 1 M sodium hydroxide.
-
-
-	* A concentration of 30 mM adenosine free base (amresco, MW 267.24 g/mol) was made from 0.0802 g of the white, needle-like solid dissolved into 10 mL of sodium phosphate buffer. However, this solution was saturated preventing the complete dissolution of adenosine.
-	* After preparing a 15 mM insoluble solution of adenosine, it was decided to prepare a 5 mM solution of adenosine. This was achieved by dissolving 0.0402 g of adenosine in 30 mL of the sodium phosphate buffer. This step was completed during the following lab period.
-
-
-	* After the use of reagents, the chemical and the newly-made stock solutions were refrigerated.
-
-

Mary Mendoza: /* Preparation of ADA Kinetic Assay Reactants */

2013-02-08T15:49:37Z

Preparation of ADA Kinetic Assay Reactants

←Older revision		Revision as of 15:49, 8 February 2013
Line 7:		Line 7:
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==Preparation of ADA Kinetic Assay Reactants==		==Preparation of ADA Kinetic Assay Reactants==
-	* ~~Agenda: To~~ prepare ADA Kinetic Assay reagents	+	* The agenda is to prepare ADA Kinetic Assay reagents for next week's Kinetic Assay runs.
	* Adenosine deaminase was ordered from Worthington (19.8 u/mg). To obtain 0.5 M per mL, the calculation below was made amounting to 0.0253.		* Adenosine deaminase was ordered from Worthington (19.8 u/mg). To obtain 0.5 M per mL, the calculation below was made amounting to 0.0253.

Mary Mendoza: /* Preparation of ADA Kinetic Assay Reactants */

2013-02-08T15:45:43Z

Preparation of ADA Kinetic Assay Reactants

←Older revision		Revision as of 15:45, 8 February 2013
Line 7:		Line 7:
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==Preparation of ADA Kinetic Assay Reactants==		==Preparation of ADA Kinetic Assay Reactants==
		+	* Agenda: To prepare ADA Kinetic Assay reagents
	* Adenosine deaminase was ordered from Worthington (19.8 u/mg). To obtain 0.5 M per mL, the calculation below was made amounting to 0.0253.		* Adenosine deaminase was ordered from Worthington (19.8 u/mg). To obtain 0.5 M per mL, the calculation below was made amounting to 0.0253.

Mary Mendoza: /* Preparation of ADA Kinetic Assay Reactants */

2013-02-05T16:05:59Z

Preparation of ADA Kinetic Assay Reactants

←Older revision		Revision as of 16:05, 5 February 2013
Line 15:		Line 15:
	* For convenience, it was decided to dissolve 2.5 mg into 100 mL of phosphate buffer. The final amount weighed 2.0 mg which will be dissolved into 80 mL of phosphate buffer. ADA was refrigerated between 2-8°C temporarily.		* For convenience, it was decided to dissolve 2.5 mg into 100 mL of phosphate buffer. The final amount weighed 2.0 mg which will be dissolved into 80 mL of phosphate buffer. ADA was refrigerated between 2-8°C temporarily.

-	* In preparation of the sodium phosphate buffer, 3.1093 g of sodium phosphate monobasic (chempure, FW 268.07 g/mol) and 10.9019 g of sodium phosphate dibasic (fisher scientific, FW 137.99 g/mol) were dissolved in 1 L water.	+	* In preparation of the sodium phosphate buffer, 3.1093 g of sodium phosphate monobasic (chempure, FW 268.07 g/mol) and 10.9019 g of sodium phosphate dibasic (fisher scientific, FW 137.99 g/mol) were dissolved in 1 L water to obtain a 0.1 M sodium phosphate buffer solution.
	* The pH of the buffer was adjusted to 7.4 by the addition of 1 M sodium hydroxide.		* The pH of the buffer was adjusted to 7.4 by the addition of 1 M sodium hydroxide.
		+
		+
		+	* A concentration of 30 mM adenosine free base (amresco, MW 267.24 g/mol) was made from 0.0802 g of the white, needle-like solid dissolved into 10 mL of sodium phosphate buffer. However, this solution was saturated preventing the complete dissolution of adenosine.
		+	* After preparing a 15 mM insoluble solution of adenosine, it was decided to prepare a 5 mM solution of adenosine. This was achieved by dissolving 0.0402 g of adenosine in 30 mL of the sodium phosphate buffer. This step was completed during the following lab period.
		+
		+
		+	* After the use of reagents, the chemical and the newly-made stock solutions were refrigerated.
		+

Mary Mendoza: /* Preparation of ADA Kinetic Assay Reactants */

2013-02-05T15:56:24Z

Preparation of ADA Kinetic Assay Reactants

←Older revision		Revision as of 15:56, 5 February 2013
Line 10:		Line 10:


-	<math>\frac{0.5 units}{19.8 u/mg}</math> = 0.0253 mg	+	<math>\frac{0.5 units}{19.8 u/mg}</math> = 0.0253 mg of ADA in 1 mL phosphate buffer


	* For convenience, it was decided to dissolve 2.5 mg into 100 mL of phosphate buffer. The final amount weighed 2.0 mg which will be dissolved into 80 mL of phosphate buffer. ADA was refrigerated between 2-8°C temporarily.		* For convenience, it was decided to dissolve 2.5 mg into 100 mL of phosphate buffer. The final amount weighed 2.0 mg which will be dissolved into 80 mL of phosphate buffer. ADA was refrigerated between 2-8°C temporarily.

-	*	+	* In preparation of the sodium phosphate buffer, 3.1093 g of sodium phosphate monobasic (chempure, FW 268.07 g/mol) and 10.9019 g of sodium phosphate dibasic (fisher scientific, FW 137.99 g/mol) were dissolved in 1 L water.
		+	* The pH of the buffer was adjusted to 7.4 by the addition of 1 M sodium hydroxide.
		+
		+
		+

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Mary Mendoza: /* Entry title */

2013-02-05T15:50:31Z

Entry title

←Older revision		Revision as of 15:50, 5 February 2013
Line 6:		Line 6:
	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==Preparation of ADA Kinetic Assay Reactants==
-	* ~~Insert content here~~...	+	* Adenosine deaminase was ordered from Worthington (19.8 u/mg). To obtain 0.5 M per mL, the calculation below was made amounting to 0.0253.

		+
		+	<math>\frac{0.5 units}{19.8 u/mg}</math> = 0.0253 mg
		+
		+
		+	* For convenience, it was decided to dissolve 2.5 mg into 100 mL of phosphate buffer. The final amount weighed 2.0 mg which will be dissolved into 80 mL of phosphate buffer. ADA was refrigerated between 2-8°C temporarily.
		+
		+	*

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Mary Mendoza: Autocreate 2013/01/29 Entry for User:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II

2013-02-02T20:24:13Z

Autocreate 2013/01/29 Entry for User:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


|}

__NOTOC__