User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/01/29: Difference between revisions
From OpenWetWare
Mary Mendoza (talk | contribs) (Autocreate 2013/01/29 Entry for User:Mary_Mendoza/Notebook/CHEM572_Exp._Biological_Chemistry_II) |
Mary Mendoza (talk | contribs) |
||
Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==Preparation of ADA Kinetic Assay Reactants== | ||
* | * Adenosine deaminase was ordered from Worthington (19.8 u/mg). To obtain 0.5 M per mL, the calculation below was made amounting to 0.0253. | ||
<math>\frac{0.5 units}{19.8 u/mg}</math> = 0.0253 mg | |||
* For convenience, it was decided to dissolve 2.5 mg into 100 mL of phosphate buffer. The final amount weighed 2.0 mg which will be dissolved into 80 mL of phosphate buffer. ADA was refrigerated between 2-8°C temporarily. | |||
* | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 08:50, 5 February 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Preparation of ADA Kinetic Assay Reactants
|