User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/01/23 - Revision history

Mary Mendoza: /* Calculation of reagents for ADA kinetic assay */

Mary Mendoza — Wed, 08 May 2013 22:09:50 GMT

Calculation of reagents for ADA kinetic assay

←Older revision		Revision as of 22:09, 8 May 2013
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-	~~==Calculation of reagents for ADA kinetic assay==~~
-	* It was decided to prepare a 30 mM stock solution of adenosine. The calculation below shows the amount needed to obtain 30 mM of adenosine.
-
-	~~.030 <math>\frac{mol}{L}</math> mol of adenosine × <math>\frac{267.24 g}{1 mol}</math> = <math>\frac{8.0172 g}{L}</math> × .010 L = 0.0802 g of adenosine~~
-
-
-	~~The calculations on the bottom are courtesy of [[User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/01/23\|Dhea Patel]].~~
-
-	~~[[Image:ADA_kinetics_table.png\|px80]]~~
-
-
-	~~The calculation of for the buffer was formulated by [[User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/01/23\|Catherine Koenigsknecht]].~~
-
-	* Procedure: Buffer
-	~~#Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)~~
-	~~#Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L. The pH of the final solution will be 7.4. This buffer can be stored for up to 1 mo at 4°C.~~
-	~~#Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)~~

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Mary Mendoza: /* Calculation of reagents for ADA kinetic assay */

Mary Mendoza — Wed, 23 Jan 2013 20:34:04 GMT

Calculation of reagents for ADA kinetic assay

←Older revision		Revision as of 20:34, 23 January 2013
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-	The calculations on the bottom are courtesy of Dhea Patel.	+	The calculations on the bottom are courtesy of [[User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/01/23\|Dhea Patel]].

	[[Image:ADA_kinetics_table.png\|px80]]		[[Image:ADA_kinetics_table.png\|px80]]


-	The calculation of for the buffer was formulated by [[User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/01/23\|Catherine Koenigsknecht]]	+	The calculation of for the buffer was formulated by [[User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/01/23\|Catherine Koenigsknecht]].

	* Procedure: Buffer		* Procedure: Buffer

Mary Mendoza: /* Calculation of reagents for ADA kinetic assay */

Mary Mendoza — Wed, 23 Jan 2013 20:31:38 GMT

Calculation of reagents for ADA kinetic assay

←Older revision		Revision as of 20:31, 23 January 2013
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	.030 <math>\frac{mol}{L}</math> mol of adenosine × <math>\frac{267.24 g}{1 mol}</math> = <math>\frac{8.0172 g}{L}</math> × .010 L = 0.0802 g of adenosine		.030 <math>\frac{mol}{L}</math> mol of adenosine × <math>\frac{267.24 g}{1 mol}</math> = <math>\frac{8.0172 g}{L}</math> × .010 L = 0.0802 g of adenosine
		+
		+
		+	The calculations on the bottom are courtesy of Dhea Patel.

	[[Image:ADA_kinetics_table.png\|px80]]		[[Image:ADA_kinetics_table.png\|px80]]

		+
		+	The calculation of for the buffer was formulated by [[User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/01/23\|Catherine Koenigsknecht]]
		+
		+	* Procedure: Buffer
		+	#Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)
		+	#Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L. The pH of the final solution will be 7.4. This buffer can be stored for up to 1 mo at 4°C.
		+	#Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Mary Mendoza: /* Calculation of reagents for ADA kinetic assay */

Mary Mendoza — Wed, 23 Jan 2013 20:27:31 GMT

Calculation of reagents for ADA kinetic assay

←Older revision		Revision as of 20:27, 23 January 2013
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	.030 <math>\frac{mol}{L}</math> mol of adenosine × <math>\frac{267.24 g}{1 mol}</math> = <math>\frac{8.0172 g}{L}</math> × .010 L = 0.0802 g of adenosine		.030 <math>\frac{mol}{L}</math> mol of adenosine × <math>\frac{267.24 g}{1 mol}</math> = <math>\frac{8.0172 g}{L}</math> × .010 L = 0.0802 g of adenosine
		+
		+	[[Image:ADA_kinetics_table.png\|px80]]

Mary Mendoza: /* Calculation of reagents for ADA kinetic assay */

Mary Mendoza — Wed, 23 Jan 2013 20:23:10 GMT

Calculation of reagents for ADA kinetic assay

←Older revision		Revision as of 20:23, 23 January 2013
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	* It was decided to prepare a 30 mM stock solution of adenosine. The calculation below shows the amount needed to obtain 30 mM of adenosine.		* It was decided to prepare a 30 mM stock solution of adenosine. The calculation below shows the amount needed to obtain 30 mM of adenosine.

-	.030 <math>\frac{mol}{L}</math> mol of adenosine × <math>\frac{267.24 g}{1 mol}</math> = <math>\frac{8.0172 g}{L}</math> × .010 L = 0.0802 g	+	.030 <math>\frac{mol}{L}</math> mol of adenosine × <math>\frac{267.24 g}{1 mol}</math> = <math>\frac{8.0172 g}{L}</math> × .010 L = 0.0802 g of adenosine

Mary Mendoza: /* Calculation of reagents for ADA kinetic assay */

Mary Mendoza — Wed, 23 Jan 2013 20:22:22 GMT

Calculation of reagents for ADA kinetic assay

←Older revision		Revision as of 20:22, 23 January 2013
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	* It was decided to prepare a 30 mM stock solution of adenosine. The calculation below shows the amount needed to obtain 30 mM of adenosine.		* It was decided to prepare a 30 mM stock solution of adenosine. The calculation below shows the amount needed to obtain 30 mM of adenosine.

-	.030 <math>\frac{mol}{L}</math> of adenosine × <math>\frac{267.24 g}{1 mol}</math>	+	.030 <math>\frac{mol}{L}</math> mol of adenosine × <math>\frac{267.24 g}{1 mol}</math> = <math>\frac{8.0172 g}{L}</math> × .010 L = 0.0802 g

Mary Mendoza: /* Calculation of reagents for ADA kinetic assay */

Mary Mendoza — Wed, 23 Jan 2013 20:05:31 GMT

Calculation of reagents for ADA kinetic assay

←Older revision		Revision as of 20:05, 23 January 2013
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	* It was decided to prepare a 30 mM stock solution of adenosine. The calculation below shows the amount needed to obtain 30 mM of adenosine.		* It was decided to prepare a 30 mM stock solution of adenosine. The calculation below shows the amount needed to obtain 30 mM of adenosine.

-	~~30 mM~~<math>\frac{mol}{L}</math> of adenosine	+	.030 <math>\frac{mol}{L}</math> of adenosine × <math>\frac{267.24 g}{1 mol}</math>

Mary Mendoza: /* Calculation of reagents for ADA kinetic assay */

Mary Mendoza — Wed, 23 Jan 2013 20:03:45 GMT

Calculation of reagents for ADA kinetic assay

←Older revision		Revision as of 20:03, 23 January 2013
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	* It was decided to prepare a 30 mM stock solution of adenosine. The calculation below shows the amount needed to obtain 30 mM of adenosine.		* It was decided to prepare a 30 mM stock solution of adenosine. The calculation below shows the amount needed to obtain 30 mM of adenosine.

-	30 mM<math>/frac{mol}{L}</math> of adenosine	+	30 mM<math>\frac{mol}{L}</math> of adenosine

Mary Mendoza: /* Calculation of reagents for ADA kinetic assay */

Mary Mendoza — Wed, 23 Jan 2013 20:03:29 GMT

Calculation of reagents for ADA kinetic assay

←Older revision		Revision as of 20:03, 23 January 2013
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	* It was decided to prepare a 30 mM stock solution of adenosine. The calculation below shows the amount needed to obtain 30 mM of adenosine.		* It was decided to prepare a 30 mM stock solution of adenosine. The calculation below shows the amount needed to obtain 30 mM of adenosine.

-	<math>~~.030~~/frac{mol}{L} ~~of adenosine~~</math>	+	30 mM<math>/frac{mol}{L}</math> of adenosine

Mary Mendoza: /* Entry title */

Mary Mendoza — Wed, 23 Jan 2013 20:02:55 GMT

Entry title

←Older revision		Revision as of 20:02, 23 January 2013
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-	==~~Entry title~~==	+	==Calculation of reagents for ADA kinetic assay==
-	* ~~Insert content here~~...	+	* It was decided to prepare a 30 mM stock solution of adenosine. The calculation below shows the amount needed to obtain 30 mM of adenosine.
		+
		+	<math>.030/frac{mol}{L} of adenosine</math>