# User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/01/23

(Difference between revisions)
 Revision as of 15:23, 23 January 2013 (view source) (→Calculation of reagents for ADA kinetic assay)← Previous diff Revision as of 15:34, 23 January 2013 (view source) (→Calculation of reagents for ADA kinetic assay)Next diff → (2 intermediate revisions not shown.) Line 11: Line 11: .030  $\frac{mol}{L}$ mol of adenosine × $\frac{267.24 g}{1 mol}$ = $\frac{8.0172 g}{L}$ × .010 L = 0.0802 g of adenosine .030  $\frac{mol}{L}$ mol of adenosine × $\frac{267.24 g}{1 mol}$ = $\frac{8.0172 g}{L}$ × .010 L = 0.0802 g of adenosine + + The calculations on the bottom are courtesy of [[User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/01/23|Dhea Patel]]. + + [[Image:ADA_kinetics_table.png|px80]] + + + The calculation of for the buffer was formulated by [[User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/01/23|Catherine Koenigsknecht]]. + + * Procedure: Buffer + #Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4) + #Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L. The pH of the final solution will be 7.4. This buffer can be stored for up to 1 mo at 4°C. + #Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)

## Revision as of 15:34, 23 January 2013

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## Calculation of reagents for ADA kinetic assay

• It was decided to prepare a 30 mM stock solution of adenosine. The calculation below shows the amount needed to obtain 30 mM of adenosine.

.030 $\frac{mol}{L}$ mol of adenosine × $\frac{267.24 g}{1 mol}$ = $\frac{8.0172 g}{L}$ × .010 L = 0.0802 g of adenosine

The calculations on the bottom are courtesy of Dhea Patel.

The calculation of for the buffer was formulated by Catherine Koenigsknecht.

• Procedure: Buffer
1. Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)
2. Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L. The pH of the final solution will be 7.4. This buffer can be stored for up to 1 mo at 4°C.
3. Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)