User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/01/23

Calculation of reagents for ADA kinetic assay

• It was decided to prepare a 30 mM stock solution of adenosine. The calculation below shows the amount needed to obtain 30 mM of adenosine.

.030 mol of adenosine × = × .010 L = 0.0802 g of adenosine

The calculations on the bottom are courtesy of Dhea Patel.

The calculation of for the buffer was formulated by Catherine Koenigsknecht.

• Procedure: Buffer

1. Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4)
2. Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L. The pH of the final solution will be 7.4. This buffer can be stored for up to 1 mo at 4°C.
3. Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)