User:Mark Skaggs/Notebook/Lab Three: Microbiology and Identifying Bacteria with DNA Sequences
Objective: To observe and identify bacteria species present within transect and agar plates through visual inspection and DNA sequencing. Also, determine antibiotic resistance within bacteria. Methods:
Observations, Results, and Conclusion:
Archaea thrive in extreme conditions such as volcanoes and the Marianas trench. Due to the nature of their preferred habitat, it is improbable that archaea would be present on the agar plates. While archaea may thrive within the petri dishes and in the presence of tetracycline, they will unlikely be within the initial sample from transect 2.
The bacteria present on each of the agar plates will change over time and in different concentrations. Also, the tetracycline plates will yield different bacteria species. As time passes, some bacteria will flourish and others will diminish. This will result in varying smells and appearances.
Dilution-------Agar Type--------Colonies Counted------Conversion Factor------Colonies/ml
10^-3----------nutrient---------------lawn------------------x10^3------------------lawn
10^-5----------nutrient---------------lawn------------------x10^5------------------lawn
10^-7----------nutrient---------------lawn------------------x10^7------------------lawn
10^-9----------nutrient---------------163-------------------x10^9------------------1.6x10^11
10^-3----------nutrient + tet--------lawn------------------x10^3------------------lawn
10^-5----------nutrient + tet--------lawn------------------x10^5------------------lawn ^ 10^-7----------nutrient + tet--------189-------------------x10^7------------------1.89x10^9
10^-9----------nutrient + tet--------18--------------------x10^9-------------------1.8x10^10
