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'''The Study of the Evolution of the Volvocine Line and Transects at AU -January 14, 2015'''
'''1.21.15--Identifying Algae and Protists and Preparing and Plating Serial Dilutions'''
 
*<u>Purpose</u>:
For this lab, the purpose was to identify six organisms in our Hay Infusion culture by using the dichotomous key. My hypothesis from the previous lab was that my transect will be more abundant with protists than algae. During this lab, I will continue to test this hypothesis.
 
*<u>Materials and Methods</u>:
 
-Prepared slides of Hay Infusion Culture from the bottom and top niches.
 
-Microscope
 
-Dichotomous Key
 
-Pipette
 
1. Make a wet mount of
 
*<u>Observations and Data</u>:
 
Hay Infusion Culture Description-
 
*smell-->musky, foul, rotten
*mold on top
*looks murky and green
*water tinged brown
 
*Descriptions of Organisms and Identification-
 
Note: Organism 1 to 3 are from the bottom niche of the jar. Organisms 4 to 6 are from the top niche of the jar.
 
First organism-Transparent, one long whip-like flagella, single cell motile, ~60 um
 
Second organism-kidney-bean shaped, bubble looking cells inside, spins
 
Third organism-seaweed looking
 
Fourth organism-almost circular, white ovals moving inside, colorless
 
Fifth organism-packman like face, spins, colorless
 
Sixth organism-peapod shape, long and thin
 
 
Figure 1-[[Image:<blockquote class="twitter-tweet" lang="en"><p>Lab 2-Bio 210-Algae <a href="http://t.co/uhtUynZywD">pic.twitter.com/uhtUynZywD</a></p>&mdash; Maria Esposito (@me3126a) <a href="https://twitter.com/me3126a/status/561013049105326080">January 30, 2015</a></blockquote>
<script async src="//platform.twitter.com/widgets.js" charset="utf-8"></script>]]
 
Figure 2-[[Image:<blockquote class="twitter-tweet" lang="en"><p>Lab 2-Bio 210-Gonium <a href="http://t.co/z9df674Dz3">pic.twitter.com/z9df674Dz3</a></p>&mdash; Maria Esposito (@me3126a) <a href="https://twitter.com/me3126a/status/561015571438120960">January 30, 2015</a></blockquote>
<script async src="//platform.twitter.com/widgets.js" charset="utf-8"></script>
]]
 
Figure 3-[[Image:<blockquote class="twitter-tweet" lang="en"><p>Lab 2-Bio 210- Peranema <a href="http://t.co/jMY136tixH">pic.twitter.com/jMY136tixH</a></p>&mdash; Maria Esposito (@me3126a) <a href="https://twitter.com/me3126a/status/561016625739341824">January 30, 2015</a></blockquote>
<script async src="//platform.twitter.com/widgets.js" charset="utf-8"></script>]]
 
Figure 4-[[Image:<blockquote class="twitter-tweet" lang="en"><p>Lab 2- Bio 210- Didinium Cyst <a href="http://t.co/7JCo0qhnoM">pic.twitter.com/7JCo0qhnoM</a></p>&mdash; Maria Esposito (@me3126a) <a href="https://twitter.com/me3126a/status/561017840804040705">January 30, 2015</a></blockquote>
<script async src="//platform.twitter.com/widgets.js" charset="utf-8"></script>]]
 
Figure 5-[[Image:<blockquote class="twitter-tweet" lang="en"><p>Lab 2- Bio 210- Colpidium <a href="http://t.co/8Rd7f2cXze">pic.twitter.com/8Rd7f2cXze</a></p>&mdash; Maria Esposito (@me3126a) <a href="https://twitter.com/me3126a/status/561019567586439169">January 30, 2015</a></blockquote>
<script async src="//platform.twitter.com/widgets.js" charset="utf-8"></script>]]
 
Figure 6-[[Image:<blockquote class="twitter-tweet" lang="en"><p>Lab 2-Bio 210-Euglena <a href="http://t.co/uSf8PaXsUP">pic.twitter.com/uSf8PaXsUP</a></p>&mdash; Maria Esposito (@me3126a) <a href="https://twitter.com/me3126a/status/561019945476456448">January 30, 2015</a></blockquote>
<script async src="//platform.twitter.com/widgets.js" charset="utf-8"></script>
]]
 
 
Figure 1. This is a picture of what we identified as Algae.
 
Figure 2. This is a picture of what we identified as Gonium.
 
Figure 3. This is a picture of what we identified as Peranema.
 
Figure 4. This is a picture of what we identified as Didinium Cyst.
 
Figure 5. This is a picture of what we identified as Colpidium.
 
Figure 6. This is a picture of what we identified as Euglena.
 
 
*<u>Conclusions and Future Directions</u>
 
Faults: My group was uncertain if we identified our six organisms correctly. And it was difficult to take picture of Figure 3 and is not a clear picture of that organism. Based off of my previous hypothesis, there is more ____than ____
The third organism we assumed it was algae
With further examination it looks like the second and fifth organisms are the same organism, Gonium.
 
 
*<u>Citations</u>:Bentley, M., Walters-Conte, K., and Nancy K. Zeller. 2015. A Laboratory Manual to Accompany: General Biology II. Department of Biology, American University: Washington D.C.
 
 
 
'''1.14.15--Biological Life at AU: Observations of a Transect'''


*<u>Purpose</u>:
*<u>Purpose</u>:
To recognize how natural selection correlates with the evolution of different species and to formulate observations about my own sample niche. During this experiment, we looked to observe unicellular organisms and protists in our Hay Infusion culture. I think my Hay Infusion culture will contain evolutionary organisms in it’s soil.
At American University, I am observing Transect 3 (Tall brushes) to understand and identify the organisms that inhabit that area and the abiotic and biotic factors that affect that niche. My hypothesis is that the transect will be more abundant with protists than bacteria. We cannot tell which is more abundant in our transect, an Hay Infusion culture will be made to test this and observations with a microscope need to be made.  


*<u>Materials and Methods</u>:
*<u>Materials and Methods</u>:


-Slides of Chlamydomonas, Gonium, and Volvox
-Scientific Microscope


-Scientific Microscope
-Sample of my transect


-Sample of my transect
-50 mL conical tube


-Protoslo
-0.1 gm of dried milk


-50 mL conical tube
-Plastic Jar


-0.1 gm of dried milk
-500 mLs of deerpark water


-Plastic Jar
-Graduated cylinder


-500 mLs of deerpark water
1. I observed and drew a picture of the area of my transect and recorded the abiotic and biotic properties of my transect.


-Graduated cylinder
2. I used a 50 mL conical tube to take a soil/vegetation sample of my transect.


# First, I observed the slides of Chlamydomonas, Gonium, and Volvox.  
3. Back in the lab, my group and I measured 500 mLs of deerpark water with a graduated cylinder. And placed it in the plastic jar.  


2. Then, I recorded the number of cells, the colony size, the specialization of cells, the mechanisms of motility, and whether the specimen was isogamous or 
4. Then, my group and I placed 12 grams of the transect sample and 0.1 gm of dried milk in the plastic jar.  
oogamous for each specimen.


3. I drew pictures of how each of the specimen looked under the microscope,
5. We mixed the contents of our Hay Infusion Culture for 10 seconds and removed the lid of the jar.


4. I observed and drew a picture of the area of my transect and recorded what my transect contained.
*<u>Data and Observations</u>:


5. I used a 50 mL conical tube to take a soil/vegetation sample of my transect.
Description of Transect-
<font color="red">


6. Back in the lab, my group and I measured 500 mLs of deerpark water with a graduated cylinder. And placed it in the plastic jar.
The abiotic factors:
*snow
*dirt
*water
*metal post
*concrete


7. Then, my group and I placed 12 grams of the transect sample and 0.1 gm of dried milk  in the plastic jar.
The biotic factors:
*leaves
*bushes
*grass


8. We mixed the contents of our Hay Infusion Culture for 10 seconds and removed the lid of the jar.
Location: inbetween Bender Arena and the Amphitheater


<u>Data and Observations</u>:
Topography: near steep area </font>


Table of the Volume Line Members:  
Figure 1-[[Image:<blockquote class="twitter-tweet" lang="en"><p>Lab 1-Bio lab 210-Transect Drawing <a href="http://t.co/dCcu56WcFA">pic.twitter.com/dCcu56WcFA</a></p>&mdash; Maria Esposito (@me3126a) <a href="https://twitter.com/me3126a/status/559933498383818752">January 27, 2015</a></blockquote>
<script async src="//platform.twitter.com/widgets.js" charset="utf-8"></script>]]


Picture:[[Image:https://twitter.com/me3126a/status/55993330195614924.jpg]]
Figure 2-[[Image:<blockquote class="twitter-tweet" lang="en"><p>Lab 1-Bio 210-Google image aerial view <a href="http://t.co/LaENO5c7Bk">pic.twitter.com/LaENO5c7Bk</a></p>&mdash; Maria Esposito (@me3126a) <a href="https://twitter.com/me3126a/status/561005359402590208">January 30, 2015</a></blockquote>
This table allowed us to view and compare the observations among the organisms.
<script async src="//platform.twitter.com/widgets.js" charset="utf-8"></script>]]


Tall Brushes Transect:  
Figure 3-[[Image: <blockquote class="twitter-tweet" lang="en"><p>Lab 1-Bio 210-Side view of Transect <a href="http://t.co/EsMs9H01RU">pic.twitter.com/EsMs9H01RU</a></p>&mdash; Maria Esposito (@me3126a) <a href="https://twitter.com/me3126a/status/561008037125967872">January 30, 2015</a></blockquote>
<script async src="//platform.twitter.com/widgets.js" charset="utf-8"></script>
]]


Picture:[[Image:https://twitter.com/me3126a/status/559933498383818752]]
Figure 4-[[Image:<blockquote class="twitter-tweet" lang="en"><p>Lab 1-Bio 210-Above view of Transect <a href="http://t.co/bxLUvfUUMz">pic.twitter.com/bxLUvfUUMz</a></p>&mdash; Maria Esposito (@me3126a) <a href="https://twitter.com/me3126a/status/561008724106813440">January 30, 2015</a></blockquote>
This is my transect (tall brushes) from an aerial view and is labeled with what it consists of.
<script async src="//platform.twitter.com/widgets.js" charset="utf-8"></script>
]]


<u>Conclusions and Future Directions</u>:
Figure 1.This is an drawing of our transect (tall brushes) from an aerial view and is labeled with abiotic and biotic factors.
This data from transect supports that there is living organisms in my Hay Infusion culture. However, I am unsure about what specific organisms. Next time, I want to put more vegetation in my transect soil sample.  


Description of Transect:
Figure 2.This is a Google image of an aerial view of our transect.


<font color="red">Abiotic: snow, dirt, water, metal post, concrete
Figure 3.This is an side view of our Hay Infusion culture.
Biotic: leaves, bushes
 
Location: inbetween Bender Arena and the Amphitheater
Figure 4.This is an above view of our Hay Infusion culture.
Topography: near steep area, is not on a hill </font>


*<u>Conclusions and Future Directions</u>:
This data from transect does not support or disprove my hypothesis, I am unsure about what specific organism are in my transect. Next lab period, further examination of the incubated Hay Infusion culture will be done. 


M.E.
M.E.
*<u>Citations</u>:Bentley, M., Walters-Conte, K., and Nancy K. Zeller. 2015. A Laboratory Manual to Accompany: General Biology II. Department of Biology, American University: Washington D.C.

Revision as of 21:50, 29 January 2015

1.21.15--Identifying Algae and Protists and Preparing and Plating Serial Dilutions

  • Purpose:

For this lab, the purpose was to identify six organisms in our Hay Infusion culture by using the dichotomous key. My hypothesis from the previous lab was that my transect will be more abundant with protists than algae. During this lab, I will continue to test this hypothesis.

  • Materials and Methods:

-Prepared slides of Hay Infusion Culture from the bottom and top niches.

-Microscope

-Dichotomous Key

-Pipette

1. Make a wet mount of

  • Observations and Data:

Hay Infusion Culture Description-

  • smell-->musky, foul, rotten
  • mold on top
  • looks murky and green
  • water tinged brown
  • Descriptions of Organisms and Identification-

Note: Organism 1 to 3 are from the bottom niche of the jar. Organisms 4 to 6 are from the top niche of the jar.

First organism-Transparent, one long whip-like flagella, single cell motile, ~60 um

Second organism-kidney-bean shaped, bubble looking cells inside, spins

Third organism-seaweed looking

Fourth organism-almost circular, white ovals moving inside, colorless

Fifth organism-packman like face, spins, colorless

Sixth organism-peapod shape, long and thin


Figure 1-[[Image:

<script async src="//platform.twitter.com/widgets.js" charset="utf-8"></script>]]

Figure 2-[[Image:

<script async src="//platform.twitter.com/widgets.js" charset="utf-8"></script> ]]

Figure 3-[[Image:

<script async src="//platform.twitter.com/widgets.js" charset="utf-8"></script>]]

Figure 4-[[Image:

<script async src="//platform.twitter.com/widgets.js" charset="utf-8"></script>]]

Figure 5-[[Image:

<script async src="//platform.twitter.com/widgets.js" charset="utf-8"></script>]]

Figure 6-[[Image:

<script async src="//platform.twitter.com/widgets.js" charset="utf-8"></script> ]]


Figure 1. This is a picture of what we identified as Algae.

Figure 2. This is a picture of what we identified as Gonium.

Figure 3. This is a picture of what we identified as Peranema.

Figure 4. This is a picture of what we identified as Didinium Cyst.

Figure 5. This is a picture of what we identified as Colpidium.

Figure 6. This is a picture of what we identified as Euglena.


  • Conclusions and Future Directions

Faults: My group was uncertain if we identified our six organisms correctly. And it was difficult to take picture of Figure 3 and is not a clear picture of that organism. Based off of my previous hypothesis, there is more ____than ____ The third organism we assumed it was algae With further examination it looks like the second and fifth organisms are the same organism, Gonium.


  • Citations:Bentley, M., Walters-Conte, K., and Nancy K. Zeller. 2015. A Laboratory Manual to Accompany: General Biology II. Department of Biology, American University: Washington D.C.


1.14.15--Biological Life at AU: Observations of a Transect

  • Purpose:

At American University, I am observing Transect 3 (Tall brushes) to understand and identify the organisms that inhabit that area and the abiotic and biotic factors that affect that niche. My hypothesis is that the transect will be more abundant with protists than bacteria. We cannot tell which is more abundant in our transect, an Hay Infusion culture will be made to test this and observations with a microscope need to be made.

  • Materials and Methods:

-Scientific Microscope

-Sample of my transect

-50 mL conical tube

-0.1 gm of dried milk

-Plastic Jar

-500 mLs of deerpark water

-Graduated cylinder

1. I observed and drew a picture of the area of my transect and recorded the abiotic and biotic properties of my transect.

2. I used a 50 mL conical tube to take a soil/vegetation sample of my transect.

3. Back in the lab, my group and I measured 500 mLs of deerpark water with a graduated cylinder. And placed it in the plastic jar.

4. Then, my group and I placed 12 grams of the transect sample and 0.1 gm of dried milk in the plastic jar.

5. We mixed the contents of our Hay Infusion Culture for 10 seconds and removed the lid of the jar.

  • Data and Observations:

Description of Transect-

The abiotic factors:

  • snow
  • dirt
  • water
  • metal post
  • concrete

The biotic factors:

  • leaves
  • bushes
  • grass

Location: inbetween Bender Arena and the Amphitheater

Topography: near steep area

Figure 1-[[Image:

<script async src="//platform.twitter.com/widgets.js" charset="utf-8"></script>]]

Figure 2-[[Image:

<script async src="//platform.twitter.com/widgets.js" charset="utf-8"></script>]]

Figure 3-[[Image:

<script async src="//platform.twitter.com/widgets.js" charset="utf-8"></script> ]]

Figure 4-[[Image:

<script async src="//platform.twitter.com/widgets.js" charset="utf-8"></script> ]]

Figure 1.This is an drawing of our transect (tall brushes) from an aerial view and is labeled with abiotic and biotic factors.

Figure 2.This is a Google image of an aerial view of our transect.

Figure 3.This is an side view of our Hay Infusion culture.

Figure 4.This is an above view of our Hay Infusion culture.

  • Conclusions and Future Directions:

This data from transect does not support or disprove my hypothesis, I am unsure about what specific organism are in my transect. Next lab period, further examination of the incubated Hay Infusion culture will be done.

M.E.

  • Citations:Bentley, M., Walters-Conte, K., and Nancy K. Zeller. 2015. A Laboratory Manual to Accompany: General Biology II. Department of Biology, American University: Washington D.C.
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