User:Mar/Notebook/2007-9-20: Difference between revisions
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(New page: = Mouse embryos E16 injection with BrdU = == Goal == #Assesment of BrdU-labeled cells' migration during undisturbed ontogenesis #To be co-immunostained for BrdU and nestin == Animals == M...) |
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== Animals == | == Animals == | ||
Mothers: | Mothers: | ||
rl111b - 39g | |||
| Line 20: | Line 20: | ||
Injection regime: | Injection regime: | ||
39g and -> inj'd 0.39mL-45'-0.39mL | 39g and -> inj'd 0.39mL-45'-0.39mL | ||
i.e. total volume 2x.39 = 0.78mL | i.e. total volume 2x.39 = 0.78mL | ||
Make 1000µL from 1 * 5mg = 5mg | Make 1000µL from 1 * 5mg = 5mg | ||
Revision as of 11:48, 20 September 2007
Mouse embryos E16 injection with BrdU
Goal
- Assesment of BrdU-labeled cells' migration during undisturbed ontogenesis
- To be co-immunostained for BrdU and nestin
Animals
Mothers:
rl111b - 39g
Embryos:
Materials
- BrdU solution: 5mg/ml in saline with 0.007 N NaOH (i.e. dilute 2N NaOH 286x). NaOH usually unnecessary.
Procedure
Protocol follows the original experiment [1]. BrdU injected to a pregnant mother:
- dose 50µg/g
- single injection labels during period extending from 15min to at least 2hr
Injection regime:
39g and -> inj'd 0.39mL-45'-0.39mL
i.e. total volume 2x.39 = 0.78mL Make 1000µL from 1 * 5mg = 5mg
