User:Madeline Hartman/Notebook/Dr. Hartings Lab/2014/02/06

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0.225 mL of Mb added after 2 minutes (between readings 2 and 3) (1 mg Mb/KCl/Phosphate buffer in 1 mL MeOH)
0.225 mL of Mb added after 2 minutes (between readings 2 and 3) (1 mg Mb/KCl/Phosphate buffer in 1 mL MeOH)
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Same steps were repeated at 15°C
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Ran a trial in OceanView for Absorbance readings.

Run at 25°C set through Qpod

  1. Run A Wizard
  2. Select spectroscopy
  3. JAZ is the correct hardware device. Click next
  4. Select absorbance and click next
  5. Select integration time as 1 ms
  6. Select scans to average 10
  7. Click next
  8. Open lamp. Click lightbulb to store reference spectrum. Click next
  9. Close lamp. Click the lightbulb to store dark spectrum. Click finish
  10. Open lamp. Click save graph to files. Configure
  11. Save the first available scan every 1 minute
  12. Stop after 2 hours
  13. Save to directory in a dropbox folder - 02-06- MeOH-25
  14. File format- Tab separated with headers
  15. Create file name - mb_opd_phosphate_meoh_h202_25
  16. Click apply
  17. When ready to run, click play button, then click the save graph to files button a second time.

Cuvette at start time contains-

  1. 0.675 mL (3.4 mg OPD in 3.4 mL MeOH)
  2. 0.75 mL dilute H2O2 (0.1135 μL H2O2 and 0.8865 μL H2O)

First reading taken after 45 seconds.

0.225 mL of Mb added after 2 minutes (between readings 2 and 3) (1 mg Mb/KCl/Phosphate buffer in 1 mL MeOH)


Same steps were repeated at 15°C


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